Iva Zahija (Author), Barbara Jeršek (Author), Lea Demšar (Author), Mateja Lušnic Polak (Author), Tomaž Polak (Author)

Abstract

The aim of the present study was to develop meat-based media with compositions similar to those of dry-fermented meat products and to evaluate their use in studying the growth of Aspergillus parasiticus and the kinetics of aflatoxin B1 (AFB1) production. In our previous experiments, we found that the strain A. parasiticus ŽMJ7 produced a high amount of AFB1. Cooked meat agar (CMA2) was used as a novel complex meat-based medium with four variations: CMA2G (CMA2 supplemented with 1% glucose), CMA2YE (CMA2 supplemented with 0.2% yeast extract), and CMA2GYE (CMA2 supplemented with 1% glucose and 0.2% yeast extract). Media were inoculated with an A. parasiticus spore suspension (105 spores/mL) and incubated at 25 °C for up to 15 days. The A. parasiticus lag phase lasted less than 1 day, irrespective of the growth medium, with the exception of control medium CMA1 (cooked meat agar) as an already known meat-based medium. The highest mean colony growth rate was observed on CMA2 and CMA2G. Reversed-phase UPLC–MS/MS analysis was performed to determine the AFB1 concentration in combination with solid phase extraction (SPE). The highest AFB1 concentration in meat-based media was detected in CMA2GYE after 15 days of incubation (13,502 ± 2367 ng/mL media). The results showed that for studying AFB1 production in dry-fermented meat products, novel suitable media such as CMA2-based media are required. This finding could represent a potential concern with regard to the production of dry-fermented meat products.

Keywords

plesni;mikotoksini;aflatoksin B1;mikrobiološka gojišča;moulds;Aspergillus parasiticus;mycotoxins;aflatoxin B1;meat-based media;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
UDC: 579.24:615.9:582.282.123.4
COBISS: 135836163 Link will open in a new window
ISSN: 2072-6651
Views: 8
Downloads: 1
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Other data

Secondary language: Slovenian
Secondary keywords: plesni;mikotoksini;aflatoksin B1;mikrobiološka gojišča;
Type (COBISS): Article
Pages: str. 1-17
Volume: ǂVol. ǂ15
Issue: ǂiss. ǂ1
Chronology: 2023
DOI: 10.3390/toxins15010025
ID: 18937220