diplomsko delo
Alesia Mevlja (Author), Tjaša Lukan (Mentor)

Abstract

Krompirjev virus Y ogroža kmetijsko pomembne rastline, kot so krompir, tobak, paprika in paradižnik. Da bi se njegov vpliv zmanjšal, je pomembno razumevanje interakcij med virusom in rastlino. Ko virus pride v stik z rastlino, ga rastlina prepozna in aktivira svoj obrambni mehanizem, ki pa ni nujno uspešen pri zaustavitvi virusa. Virus za svoje razmnoževanje in premikanje v rastlini potrebuje rastlinske faktorje. Posledično sta rastlina in virus ves čas v interakciji. Z raziskovanjem interakcij so se raziskovalci ukvarjali že v številnih študijah, vendar so številni mehanizmi kljub temu še vedno nepoznani in nerazumljeni. Ves čas prihaja do odkritja novih interakcij med proteini virusa in proteini rastline. Za sledenje virusa v rastlini in odkrivanje interakcij med proteini, lahko virus ali virusne proteine označimo s fluorescenčnimi označevalci. Informacije, ki jih pridobimo na tak način, nam omogočajo, da tarčno ciljamo na ključne virusne proteine in tako onemogočimo razmnoževanje virusa. Lahko pa z uporabo genskega inženiringa rastline spremenimo in s tem zavremo interakcije med ključnimi proteini in rastlino in s tem preprečimo širjenje in razmnoževanje virusa.

Keywords

krompirjev virus Y;PVY;interakcije virus­rastlina;orodja za preučevanje interakcij;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Mevlja]
UDC: 606:631.528:632.38(043.2)
COBISS: 162509827 Link will open in a new window
Views: 66
Downloads: 16
Average score: 0 (0 votes)
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Other data

Secondary language: English
Secondary title: Overview of tools for studying plant – potato virus Y interaction
Secondary abstract: Potato virus Y threatens agriculturally important crops such as potatoes, tobacco, peppers and tomatoes. In order to reduce its impact, it is important to understand the interactions between the virus and the plant. When a virus comes in contact with a plant, the plant recognizes it and activates its defence mechanism, which is not necessarily successful in stopping the virus. The virus needs plant factors for its reproduction and movement in the plant. As a result, the plant and the virus are constantly interacting. Researchers have already investigated interactions in numerous studies, but many mechanisms are still unknown and poorly understood. New interactions between virus proteins and plant proteins are being discovered constantly. To track the virus in the plant and detect interactions between proteins, the virus or viral proteins can be labeled with fluorescent markers. The information we obtain in this way allows us to target key viral proteins and thus disable the virus from replicating. However, we can use genetic engineering to edit plant genome and thereby suppress the interactions between key proteins and the plant, thereby preventing the spread and reproduction of the virus.
Secondary keywords: potato virus Y;virus­plant interactions;tools for studying interactions;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VI, 20 str.))
ID: 19853829