magistrsko delo
Ana Pintar (Author), Katarina Kos (Mentor), Helena Grčman (Thesis defence commission member), Franci Aco Celar (Thesis defence commission member)

Abstract

V biotičnem varstvu se za zatiranje patogenov uporablja žive organizme. Cilj te magistrske naloge je ugotoviti, kako antagonistične glive iz rodu Trichoderma, izolirane iz pripravkov Pannon Starter® Perfect Pro in Trifender® pro WP, vplivajo na rast micelija in tvorbo sklerocijev patogenih gliv – S. sclerotiorum, S. minor, S. cepivorum, A. rolfsii, M. phaseolina - in kakšne so možnosti za nadaljnjo uporabo teh biotičnih pripravkov v kmetijstvu. Kot pozitivna kontrola je bila uporabljena hiperparazitska gliva C. minitans, izolirana iz pripravka Contans® WG. Izvedli smo poskus dvojnih kultur, kjer smo v različnih terminih na gojišče inokulirali eno od patogenih gliv (0., 3. ali 7. dan) v kombinaciji z eno od antagonističnih gliv. Poskus smo izvedli pri dveh temperaturah, 15 in 25 °C. Vsak dan smo označevali dnevni prirast micelija gliv in po 10 dneh ugotovili, da temperatura različno vpliva na njihovo interakcijo. Nacepljanje patogene glive na gojišče k antagonistični glivi 3. ali 7. dan nista pokazali opaznih razlik od hkrati nacepljenih gliv na gojišče. Ugotovili smo, da je temperatura imela velik vpliv na rast micelija in tudi na interakcijo obeh gliv. Mikoparazitizem sklerocijev patogenih gliv s strani glive Trichoderma smo raziskovali na dva različna načina, in sicer z dodajanjem suspenzije pripravkov na sklerocije patogena, ki se je izkazal za bolj zanesljivega, in s kasnejšim vizualnim ocenjevanjem sklerocijev, nastalih v dvojni kulturi. V raziskavo smo vključili še lončni poskus, kjer smo sadikam solate dodajali sklerocije S. sclerotiorum in S. minor ter suspenzije prej uporabljenih pripravkov. Naša raziskava je dobra osnova za nadaljnje poskuse, pri iskanju novih biotičnih pripravkov z uporabo gliv iz rodu Trichoderma.

Keywords

Trichoderma spp.;biotično varstvo;antagonistične glive;patogene glive;sklerocij;mikoparazitizem;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [A. Pintar]
UDC: 632.937.14.05:632.4(043.2)
COBISS: 162685443 Link will open in a new window
Views: 30
Downloads: 5
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Other data

Secondary language: English
Secondary title: The study of the effect of genus Trichoderma on growth and sclerotia formation of selected pathogenic fungi
Secondary abstract: In biological control, living organisms are used for pest management. The aim of this master's thesis is to determine how antagonistic fungi from the genus Trichoderma, isolated from the biostimulants Pannon Starter® Perfect Pro and Trifender® pro WP, affect the growth of mycelium and the formation of sclerotia of pathogenic fungi - S. sclerotiorum, S. minor, S. cepivorum, A. rolfsii, M. phaseolina - and what are the possibilities for further use of biological control agents in agriculture. The hyperparasitic fungus C. minitans, isolated from Contans® WG, was used as a positive control. We performed an experiment of dual culture, where one of the pathogenic fungi was inoculated on the culture medium at different times (day 0, day 3 or day 7) in combination with one of the antagonistic fungi. The experiment was conducted at two temperatures, 15 and 25 °C. For 10 days we marked the daily growth of mycelium of fungi. We found that the temperature had a great influence on the growth of the mycelium and also on the interaction of the two fungi Inoculation of the pathogenic fungus on the culture medium to the antagonistic fungus on day 3 or day 7 did not show any visible difference from 0. day inoculation of fungi on medium. Mycoparasitism of sclerotia of pathogenic fungi by the Trichoderma spp. was researched in two different ways. First by adding a suspension of biostimulants to the sclerotia of the pathogen, which proved to be more reliable, and second, by subsequent visual evaluation of sclerotia formed in dual culture. In addition, we included a pot experiment, where sclerotia of S. sclerotiorum and S. minor and suspensions of previously used biostimulants were added to lettuce seedlings. Our research is a good basis for further experiments to search new biotic protection products using fungi of the Trichoderma genus.
Secondary keywords: biological control;Trichoderma spp.;antagonistic fungus;pathogenic fungus;sclerotium;mycoparasitism;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za agronomijo
Pages: 1 spletni vir (1 datoteka PDF (XII, 47 str., [4] str. pril.))
ID: 19867805