diplomsko delo
Vanja Ivošević (Author), Vera Župunski (Mentor)

Abstract

Pri bolezni amiotrofična lateralna skleroza je najpogostejša mutacija povečanje števila ponovitev GGGGCC tudi do tisočkrat v intronu gena C9orf72. V plazmid pRINT se vnese zapis za smerne in protismerne ponovitve GGGGCC. Plazmid zapisuje rdeči fluorescenčni protein. V intron se lahko vnese zapis za željeni protein in tako nastane reporterski sistem, ki dokazuje, da se zapis za željeni protein (v tem primeru ponovitve GGGGCC) res prepisuje. Nastane mRNA in intron, v katerem so ponovitve ali zapis za željeni protein, se izreže. Delovanje reporterskega sistema se potrdi tudi z detekcijo ponovitev v celicah.

Keywords

heksanukleotidne ponovitve GGGGCC;C9orf72;vektor pRINT;mutageneza vektorja;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [V. Ivošević]
UDC: 577.21(043.2)
COBISS: 165233667 Link will open in a new window
Views: 34
Downloads: 5
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Other data

Secondary language: English
Secondary title: Cloning of sense and antisense GGGGCC repeats into the pRINT vector and their detection in mammalian cell cultures
Secondary abstract: The most common mutation detected in amyotrophic lateral sclerosis is an increase in the number of GGGGCC repeats up to a thousand times in the intron of the C9ORF72 gene. We will insert sense and antisense GGGGCC repeats into the intron of red fluorescence protein in the pRINT plasmid. This way a reporter system is formed, which confirms that GGGGCC repeats are transcribed if the cells are red.
Secondary keywords: amyotrophic lateral sclerosis;hexanucleotide repeats GGGGCC;pRINT vector;Molekularno kloniranje;Amiotrofična lateralna skleroza;Univerzitetna in visokošolska dela;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000371
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Biokemija
Pages: 34 str.
ID: 19876321