Protiglivna aktivnost izvlečkov navadne in krhljaste barvilnice

magistrsko delo

Natalija Gruden (Author), Simona Strgulc-Krajšek (Mentor), Sabina Anžlovar (Co-mentor)

Abstract

V magistrskem delu smo proucevali protiglivno aktivnost izvleckov, pridobljenih iz posušenih listov, korenov in plodov dveh v Sloveniji invazivnih rastlinskih vrst – navadne barvilnice (Phytolacca americana) in krhljaste barvilnice (P. acinosa). Izvlecke smo pripravili z ekstrakcijo z etanolom in testirali njihovo protiglivno delovanje na vrste Alternaria alternata, Alternaria infectoria, Aspergillus flavus, Epicoccum nigrum, Botrytis cinerea, Fusarium poae in Fusarium oxysporum. Pripravili smo 10-% etanolne izvlecke, ki smo jih dodali na 2-% PDA gojišce in na njih neposredno nanesli kulturo glive. Vse tretmaje smo trikrat ponovili. Nekateri izvlecki so rast gliv zavirali, nekateri pospeševali. Najvecji zaviralni ucinek na rast gliv sta imela izvlecka iz listov P. acinosa in plodov P. americana. S pomocjo teh izvleckov smo kot dokaz o protiglivnem delovanju nadaljevali raziskave in proucevali glive, ki so imele 60-% ali vecji zaviralni ucinek na rast micelija. Za izvlecke sta bili najbolj obcutljivi dve glivi – Botrytis cinerea in Aspergillus flavus. Obe glivi smo gojili na PDA gojišcu z izvlecki s koncentracijami 25 %, 12,5 %, 6,2 %, 3,1 %, 1,6 %, 0,8 % in ugotovili, da je bila inhibicija rasti koncentracijsko odvisna. Rezultati so pokazali, da bi bili lahko izvlecki, pridobljeni iz listov P. acinosa in plodov P. americana, potencialno uporabni kot biofungicidi v kmetijstvu in kot alternativa sinteticnim fungicidom pri zaviranju rasti škodljivih gliv v živilski industriji.

Keywords

barvilnica;navadna barvilnica;Phytolacca americana;krhljasta barvilnica;P. acinosa;glive;protiglivna aktivnost;inhibicija rasti;

Data

Language:	Slovenian
Year of publishing:	2023
Typology:	2.09 - Master's Thesis
Organization:	UL PEF - Faculty of Education
Publisher:	[N. Gruden]
UDC:	58(043.2)
COBISS:	163138307
Views:	56
Downloads:	3
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Antifungal activity of Phytolacca americana and P. acinosa extracts
Secondary abstract:	In the master's thesis, we investigated the antifungal activity of extracts obtained from dried leaves, roots, and fruits of two invasive plant species in Slovenia - Phytolacca americana and P. acinosa. Extracts were prepared by ethanol extraction and tested for their antifungal activity against the following species: Alternaria alternata, Alternaria infectoria, Aspergillus flavus, Epicoccum nigrum, Botrytis cinerea, Fusarium poae and Fusarium oxysporum. We prepared 10% ethanol extracts, which were added to 2% PDA medium and fungal culture was directly applied to them. All treatments were repeated three times. Some extracts were shown to inhibit the growth of fungi, some to accelerate it. Extracts from P. acinosa leaves and P. americana fruits had the greatest inhibitory effect on fungal growth. With the help of these extracts as evidence of antifungal activity, we continued research and studied fungi that had a 60% or greater inhibitory effect on the growth of mycelium. Botrytis cinerea and Aspergillus flavus were the two most sensitive fungi to the extracts. Both fungi were grown on PDA medium with extracts at concentrations of 25%, 12.5%, 6.2%, 3.1%, 1.6%, 0.8% and we found that growth inhibition was concentration-dependent. The results showed that extracts obtained from P. acinosa leaves and P. americana fruits could potentially be used as biofungicides in agriculture and as alternatives to synthetic fungicides for inhibiting the growth of harmful fungi in the food industry.
Secondary keywords:	pokeweed;Phytolacca americana;P. acinosa;fungi;antifungal efficiency;growth inhibiton;Glive;Botanika;Univerzitetna in visokošolska dela;
Type (COBISS):	Master's thesis/paper
Study programme:	0
Thesis comment:	Univ. v Ljubljani, Pedagoška fak., Poučevanje
Pages:	1 spletni vir (1 datoteka PDF (XVI, 70 str.))
DOI:	20.500.12556/RUL-149109
ID:	19884057