diplomsko delo
Rok Kozamernik (Author), Polona Žnidaršič Plazl (Mentor)

Abstract

Encimi so biokatalizatorji, ki omogočajo trajnostno izvedbo številnih industrijsko pomembnih reakcij. V reakcijski mešanici so encimi lahko raztopljeni, lahko pa jih imobiliziramo. Prednosti imobilizacije so povečana stabilnost encimov, podaljšanje časa njihovega delovanja, možnost ponovne uporabe encimov in močno olajšanje separacijskih procesov po katalitski reakciji. Implementacija encimske imobilizacije je obetavna tudi na področju mikrobioreaktorjev, ki so miniaturizirani reaktorji, v katerih potekajo biotransformacije. Kot dobre nosilne strukture za imobilizacijo encimov v mikrobioreaktorjih so se nanomateriali izkazali predvsem zaradi svoje velike specifične površine in svoje majhnosti. Več encimom dostopne površine pomeni večjo koncentracijo encimov v reaktorju, kar lahko vodi do bolj učinkovitih biokatalitskih procesov. Pomembne lastnosti nanomaterialov kot nosilcev encimov so biološka kompatibilnosti, netoksičnost in možnost njihovega modificiranja. V tem delu se osredotočam na magnetne nanodelce, nanovzmeti, nanotkanine in kovinsko-organska ogrodja. Obravnavam tudi konkretne primere uporabe naštetih nanomaterialov, kot so deaminacija 1-fenilalanina z encimsko prevlečenimi magnetnimi nanodelci, imobilizacija β-galaktozidaze na nanovzmeti iz sillicijevega dioksida, imobilizacija lipaze na nanovlakna iz polilaktične kisline in imobilizacija lipaze na kovinsko-organsko ogrodje za proizvodnjo varfarina.

Keywords

biokataliza;biokatalitski procesi;imobilizacija encimov;mikrobioreaktorji;diplomska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [R. Kozamernik]
UDC: 66.098:620.3(043.2)
COBISS: 169993219 Link will open in a new window
Views: 59
Downloads: 4
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Other data

Secondary language: English
Secondary title: The use of nanomaterials for immobilization of enzymes in microreactors
Secondary abstract: Enzymes are biocatalysts that enable the sustainable execution of numerous industrially significant reactions. In the reaction mixture, enzymes can be dissolved, or they can be immobilized. The advantages of immobilization include increased enzyme stability, extended operational lifetimes, enzyme reusability, and significant facilitation of separation processes after catalytic reactions. The implementation of enzyme immobilization also shows promise in the field of microbioreactors, which are miniaturized reactors in which biotransformations take place. Nanomaterials have proven to be excellent solid supports for enzyme immobilization in microbioreactors, primarily due to their large specific surface area and small size. A greater surface area accessible to enzymes results in a higher enzyme concentration in the reactor, potentially leading to more efficient biocatalytic processes. Important characteristics of nanomaterials as enzyme carriers include biological compatibility, non-toxicity and the potential for modification. In this work, I focus on magnetic nanoparticles, nanosprings, nanofibers, and metal-organic frameworks. I also discuss specific examples of using the listed nanomaterials, such as the deamination of 1-phenylalanine with enzyme-coated magnetic nanoparticles, immobilization of β-galactosidase on a silicon dioxide nanospring, immobilization of lipase on polylactic acid nanofibers, and immobilization of lipase on a metal-organic framework for warfarin production.
Secondary keywords: biocatalysis;enzyme immobilization;microbioreactors;nanomaterials;Nanostrukturni materiali;Encimi;Mikroreaktorji;Univerzitetna in visokošolska dela;
Type (COBISS): Bachelor thesis/paper
Study programme: 1000372
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, UNI Kemijsko inženirstvo
Pages: 33 str.
ID: 19913005