Viabilnost semen kukavičevk iz semenske banke Botaničnega vrta v Ljubljani in njihova asimbiotska kalitev na primeru semen lepega čeveljca (Cypripedium calceolus L.)

diplomsko delo

Monika Kaplja (Author), Jana Ambrožič-Dolinšek (Mentor)

Abstract

V diplomskem delu smo preverjali živost semen kukavičevk, nabranih na področju Slovenije in shranjenih v semenski banki Botaničnega vrta v Ljubljani. Za preverjanje živosti 21 vrst kukavičevk, nabranih med leti 2007 in 2012, smo preizkusili in primerjali dve metodi: metodo s terazolijevim kloridom (TTC) in fluorescein diacetatom (FDA). Metoda FDA se je izkazala za primernejšo; rezultati živosti semen so bili zelo variabilni in so znašali od 0,5 do 100 %. Da bi preverili ali živost semen pomeni tudi kalivost, smo se odločili, da kalimo eno izmed posredovanih kukavičevk tudi aseptično kalimo. Izbrali smo kukavičevko lepi čeveljc. Čeprav smo preizkusili 3 različna gojišča, nobeno od semen ni uspešno kalilo.

Keywords

biologija;lepi čeveljc;semenska banka;kalitev;diplomska dela;

Data

Language:	Slovenian
Year of publishing:	2012
Source:	Maribor
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	[M. Kaplja]
UDC:	57/59(043.2)
COBISS:	19186952
Views:	2075
Downloads:	126
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Seed viability of orchidaceae seeds from Botanical garden of Ljubljana seed bank and their asymbiotic germination on the case of Yellow lady's slipper orchid seed (Cypripeduim calceolus L.)
Secondary abstract:	We tested the viability of orchid seeds from Bpotanical garden of Ljubljana, Slovenia. The viability of 21 orchidaceae species taken between years 2007 and 2010 were tested with two different methods; the tetrazolium chloride (TTC) and the fluorescein diacetat (FDA) method. Due to particularly small seeds the FDA method reached better than TTC. The seed viability vary from o,5 to 100 %. We also checked whether viability also leads to successful germination. We chose commericially interesting Lady's Slipper Orchid (Cypripeduim calceolus L.), however without successful germination.
Secondary keywords:	seed bank;viability;FDA;asymbiotic germination;Lady's Slipper Orchid.;
URN:	URN:SI:UM:
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. v Mariboru, Fakulteta za naravoslovje in matematiko, Oddelek za biologijo
Pages:	92 f.
Keywords (UDC):	mathematics;natural sciences;naravoslovne vede;matematika;biological sciences in general;biologija;
ID:	19981