doktorska disertacija
Jure Mravlje (Author), Katarina Vogel-Mikuš (Mentor)

Abstract

Semena rastlin pogosto okužujejo glive, bodisi na polju ali med skladiščenjem. Te glive lahko povzročajo številne rastlinske bolezni, mnoge med njimi pa so sposobne tvoriti mikotoksine, ki so nevarni za ljudi in živali. Glivne okužbe tako močno ogrožajo semena, s tem pa povzročajo ogromno kmetijsko škodo ter finančne izgube na svetovni ravni. Tehnologija uporabe hladne plinske plazme (HPP) je v zadnjih desetletjih zelo napredovala in ponuja učinkovite rešitve na številnih področjih, med drugim tudi na področju dekontaminacije mikroorganizmov s površine semen. V okviru tega dela smo raziskovali uporabnost kisikove nizkotlačne HPP, ki smo jo ustvarili z radiofrekvenčnim generatorjem za namene dekontaminacije gliv s površine semen navadne in tatarske ajde. Zanimalo nas je: (i) kako HPP vpliva na pogostost in diverziteto naravno prisotnih gliv na semenih ajde, (ii) kako način obdelave s HPP (neposredni ali posredni) vpliva na glivno združbo semen ajde, (iii) ali je občutljivost na HPP vrstno specifična in (iv) kako obdelava s HPP vpliva na kalitev semen ajde. Ugotovili smo, da se z naraščanjem časa obdelave s HPP postopno zmanjšuje intenziteta okužbe, pogostost in diverziteta naravno prisotnih gliv na semenih ajde, pri čemer je neposredna obdelava s HPP učinkovitejša za namene dekontaminacije gliv kot posredna. Dokazali smo, da je občutljivost gliv na obdelavo s HPP vrstno specifična. Kot najbolj odporne proti obdelavi s HPP so se izkazale glive iz rodu Fusarium, ki imajo mikrokonidije. Hkrati smo ugotovili, da daljše obdelave s HPP zelo negativno vplivajo na kaljivost semen ajde, verjetno kot posledica jedkanja zunanjih slojev semena in deloma toplotnega učinka. Tovrstna obdelava semen ajde s HPP bi bila primerna v prehranske namene, ne pa za setev.

Keywords

hladna plazma;semena;zrnje;ajda;glive;dekontaminacija;sterilizacija;rastlinski patogeni;rastlinska pridelava;doktorska disertacija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.08 - Doctoral Dissertation
Organization: UL BF - Biotechnical Faculty
Publisher: [J. Mravlje]
UDC: 57:633.12:533.91(043.3)
COBISS: 168294147 Link will open in a new window
Views: 51
Downloads: 20
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Other data

Secondary language: English
Secondary title: Effects of cold gas plasma on seed fungal microbiome of common and Tartary buckwheat
Secondary abstract: Plant seeds are often infected by fungi, either in the field or during storage. These fungi can cause plant diseases, and many can produce mycotoxins that are dangerous for humans and animals. Fungal infections are thus a serious threat, causing enormous agricultural and financial losses worldwide. Cold gas plasma (CGP) technology has advanced greatly in recent decades. It offers practical solutions in many areas, including microbial decontamination from the surface of seeds. In this work, we investigated the applicability of low-pressure oxygen CGP, obtained with a radio frequency generator, for fungal decontamination from the surface of common and Tartary buckwheat seeds. We were interested in: (i) how CGP affects: the frequency and diversity of naturally occurring fungi on buckwheat seeds, (ii) how the method of treatment with CGP (glow or afterglow) affects the fungal community of buckwheat seeds, (iii) whether the sensitivity to CGP is species-specific and (iv) how CGP treatment affects buckwheat seed germination. We found that as the treatment time with CGP increases, the infection rate, frequency and diversity of naturally occurring fungi on buckwheat seeds gradually decreases, whereby treatment in glow CGP mode is more effective than in afterglow. We demonstrated that the sensitivity of fungi to CGP treatment is species-specific. Fungi from the genus Fusarium, which have microconidia, proved to be the most resistant to CGP treatment. We also found that longer treatments with CGP had a very negative effect on the germination of buckwheat seeds, probably due to the etching of the outer layers of the seed and partly due to the thermal effects. This kind of treatment of buckwheat seeds with CGP would be suitable for treatment for food and feedstock but not for further sowing.
Secondary keywords: cold plasma;seeds;grains;buckwheat;fungi;decontamination;sterilisation;plant pathogens;plant production;
Type (COBISS): Doctoral dissertation
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak.
Pages: 1 spletni vir (1 datoteka PDF (VIII, 107, [2] str.)
ID: 20020629