magistrsko delo
Patricija Krapež (Author), Martina Oder (Reviewer), Rok Fink (Mentor)

Abstract

Uvod: Z nenadzorovano uporabo sintetiziranih protimikrobnih snovi v vsakdanjem življenju so se povečale številne negativne posledice na globalno zdravje in okolje. Negativno dojemanje potrošnikov do industrijsko sintetiziranih protimikrobnih snovi je privedlo do zanimanja za uporabo naravnih snovi rastlinskega izvora s protibakterijskimi lastnostmi. Pomembno je, da se pri preučevanju protimikrobne aktivnosti naravnih snovi ne osredotočimo zgolj na planktonske mikroorganizme, ampak preučimo njihovo delovanje na bolj odporne oblike, kot so spore in biofilmi. Namen: Namen magistrskega dela je analizirati in ovrednotiti protibakterijsko delovanje izbranih aktivnih komponent eteričnih olj na odstranjevanje biofilmov bakterije Escherichia coli in Staphylococcus aureus. Metode dela: Za analizo in ovrednotenje protibakterijskega delovanja posamezne aktivne komponente eteričnega olja na odstranjevanje biofilmov smo uporabili različne koncentracije linalola in citronelala ter natrijevega hipoklorita kot standardnega dezinfekcijskega sredstva. V raziskavi smo uporabili testni bakteriji: Escherichia coli in Staphylococcus aureus. Za začetek naše raziskave smo izbranim spojinam za testni bakteriji določili minimalno inhibitorno koncentracijo in minimalno baktericidno koncentracijo. S kristal vijolično metodo in štetjem kolonij smo ugotavljali delovanje izbranih aktivnih komponent in natrijevega hipoklorita proti biofilmom izbranih testnih bakterij na podlagi količine biomase in števila preživelih celic. Rezultati: Rezultati so pokazali inhibitorno in baktericidno delovanje linalola, citronelala in natrijevega hipoklorita na izbrani testni bakteriji Escherichia coli in Staphylococcus aureus. Optična gostota biofilma in število preživelih celic uporabljenih bakterij, z višjo koncentracijo izbrane komponente padata. Razprava in zaključek: Ugotovili smo, da imata naravni protimikrobni snovi linalol in citronelal dober potencial pri odstranjevanju biofilma bakterije Escherichia coli in Staphylococcus aureus in sta tako primerljivi s standardno protimikrobno snovjo natrijevim hipokloritom. Za zmanjšanje negativnih posledic na globalno zdravje in okolje so potrebne nadaljnje raziskave naravnih protimikrobnih snovi pri odstranjevanju biofilmov s površin.

Keywords

magistrska dela;sanitarno inženirstvo;eterična olja;aktivne komponente;linalol;citronelal;protibakterijsko delovanje;biofilm;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL ZF - University College of Health Studies
Publisher: [P. Krapež]
UDC: 614
COBISS: 169085443 Link will open in a new window
Views: 10
Downloads: 4
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Other data

Secondary language: English
Secondary title: ǂThe ǂeffect of linalool, citronellal and sodium hypochlorite on E. coli and S. aureus biofilms
Secondary abstract: Introduction: With the uncontrolled use of synthesized antimicrobial substances in everyday life, numerous negative consequences for global health and the environment have increased. A negative perception of consumers towards industrially synthesized antimicrobial substances has led to an interest in the use of natural plant-based substances with antibacterial properties. It is important that when studying the antimicrobial activity of natural substances, we do not focus solely on planktonic microorganisms but also examine their effects on more resistant forms, such as spores and biofilms. Purpose: The purpose of the master’s thesis is to analyze and evaluate the antibacterial action of selected active components of essential oils on the removal of biofilms bacteria Escherichia coli and Staphylococcus aureus. Methods: We used different concentrations of linalool, citronellal, and sodium hypochlorite to analyze and evaluate the antibacterial action of individual active components of essential oil on biofilm removal. In our research, we used test bacteria: Escherichia coli and Staphylococcus aureus. To start our research, we determined the minimum inhibitory concentration and minimum bactericidal concentration for the selected compounds for the test bacteria. We used the crystal violet method and colony counting to determine the activity of selected active components and sodium hypochlorite against biofilms of selected test bacteria based on biomass quantity and number of surviving cells. Results: The results showed inhibitory and bactericidal effects of linalool, citronellal, and sodium hypochlorite on the selected test bacteria Escherichia coli and Staphylococcus aureus. The optical density and number of surviving cells of the biofilm bacteria Escherichia coli and Staphylococcus aureus decrease with higher concentrations of the selected component. Discussion and conclusion: We found that natural antimicrobial substances linalool and citronellal have good potential in removing biofilm of the bacteria Escherichia coli and Staphylococcus aureus and are comparable to the standard antimicrobial substance sodium hypochlorite. Further research on natural antimicrobial substances is needed to reduce negative effects on global health and the environment when removing biofilms from surfaces.
Secondary keywords: master's theses;sanitary engineering;essential oils;active components;linalool;citronellal;antibacterial activity;biofilm;
Type (COBISS): Master's thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Zdravstvena fak., Oddelek za sanitarno inženirstvo
Pages: 40 str.
ID: 20369585