magistrsko delo
Nika Jagodic (Author), Alojz Ihan (Reviewer), Andreja Nataša Kopitar (Co-mentor)

Abstract

Novi koronavirus SARS-CoV-2 se je v zadnjih treh letih razširil po celem svetu. V sklopu magistrske naloge smo želeli na osnovi imunoloških kazalcev predvideti možnosti težjega poteka bolezni, bolj usmerjenega tarčnega zdravljenja citokinske nevihte ob okužbi s SARS-CoV-2 ter primerjanje protitelesnega odziva pri bolnikih s težjim in lažjim potekom bolezni. Odvzemi krvi za imunološke preiskave so bili izvedeni v rednih intervalih. Podatke smo obdelali v programih MS Excel in RStudio. Za testiranje normalnosti porazdelitve podatkov smo uporabili Shapiro-Wilk test. V kolikor je bila porazdelitev normalna, smo uporabili parni T test, v nasprotnem primeru smo uporabili Mann-Whitneyev U test. Lažji potek je imelo 20, težjega pa 96 bolnikov (82,8%). Koncentracije limfocitnih populacij so se pri bolnikih v akutni fazi znižane, ob kontrolnem odvzemu pa so se normalizirale. Pri monocitih smo pričakovano opazili znižano izražanje molekul HLA-DR pri bolnikih s težjim potekom bolezni v primerjavi z bolniki lažjega poteka bolezni. Koncentracija specifičnih protiteles IgA na COVID-19 je po bolezni pri bolnikih z lažjim potekom bolezni naraščala veliko krajše obdobje kot pri bolnikih s težjim potekom bolezni. Kot možne prognostične biomarkerje za težji potek bolezni bi predlagali starost nad 50 let, moški spol, pridružene bolezni, povišane vrednosti fibrinogena, D-dimera, CRP, prokalcitonina, feritina, laktatne dehidrogenaze, IL-6, povišane koncentracije belih krvničk in nevtrofilcev ter znižane koncentracije limfocitov, celic T pomagalk, citotoksičnih celic T, aktiviranih limfocitov T in aktiviranih citotoksičnih celic T.

Keywords

SARS-CoV-2;COVID-19;imunski odziv;težji potek bolezni;lažji potek bolezni;klinični znaki;limfociti;protitelesa;citokini;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [N. Jagodic]
UDC: 57.083
COBISS: 173740547 Link will open in a new window
Views: 22
Downloads: 2
Average score: 0 (0 votes)
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Other data

Secondary language: English
Secondary title: Immune response to SARS-CoV-2 in patients with severe course of COVID-19
Secondary abstract: The new coronavirus SARS-CoV-2 has spread worldwide in the last three years. Using immunological indicators, we aimed to predict the possibility of a more severe course of disease, a more targeted treatment of cytokine storm during SARS-CoV-2 infection, and a comparison of antibody response in patients with severe and mild course of the disease. Blood samples were collested at regular intervals for immunological studies. We processed the data in MS Excel and RStudio. The Shapiro-Wilk test was used to test the normality of the data distribution. If the distribution was normal, the paired T test was used; otherwise, the Mann-Whitney U test was used. Twenty patients had a mild course, and 96 patients (82.8%) had a more severe course. Concentrations of lymphocyte populations decreased in patients during the acute phase but normalized during the control examinations. In monocytes, as expected, we observed a decreased expression of HLA-DR molecules in patients with a more severe disease course compared with patients with a milder disease course. The concentration of specific IgA antibodies against COVID-19 increased in patients with a milder disease course for a much shorter period after disease than in patients with a more severe disease. As possible prognostic biomarkers for a more severe disease course, we suggest age older than 50 years, male sex, co-morbidities, increased levels of fibrinogen, D-dimer, CRP, procalcitonin, ferritin, lactate dehydrogenase, IL-6, increased concentrations of white blood cells and neutrophils, and decreased concentrations of lymphocytes, helper T cells, cytotoxic T cells, activated T lymphocytes and activated cytotoxic T cells.
Secondary keywords: SARS-CoV-2;COVID-19;immune response;severe course of the disease;milder course of the disease;clinical signs;lymphocytes;antibodies;cytokines;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za biologijo, Magistrsko delo magistrskega študijskega programa 2. stopnje Molekulske in funkcionalne biologije
Pages: 1 spletni vir (1 datoteka PDF (IX, 77 str., [8] str. pril.)
ID: 21193279