Tomaž Volčič (Author), Drago Kompan (Author), Peter Dovč (Author), Simon Horvat (Author)

Abstract

Rodovniški podatki kontroliranih tropov so bistvenega pomena za rejce ovc in izvajanje selekcijskih programov. V tej študiji smo preverili, če je mono z osmimi molekularno genetskimi označevalci ter uporabo računalniškega programa ATLAS preveriti skladnost podatkov v več-generacijskih rodovnikih jezersko-solčavske in oplemenjene jezersko-solčavske pasme. Ugotovili smo 90,9% točnost beleženja rodovniških podatkov, medtem ko smo neskladja ugotovili pri štirih družinah od 44-ih. V dveh primerih je bil potomcu nepravilno pripisan oče ali mati, pri ostalih dveh primerih pa sta bila jagnjetu napačno pripisana oba starša. V drugem delu raziskave smo poskušali z istim nizom mikrosatelitnih označevalcev določiti očetovstva štirim jagnjetom istrske pramenke, kjer v času paritev v trop pripuščajo dvajset ovnov in tako jagnje nima poznanega očeta. Trem jagnjetom smo lahko nedvoumno določili očeta z izločitvijo ostalih devetnajstih. V enem primeru smo ugotovili, da niz osmih označevalcev ni dovolj informativen za določitev očeta potomcu, zato smo analizo razširili in z vključitvijo dodatnih štirih mikrosatelitnih označevalcev uspešno določili očetovstvo tudi v tem primeru. Z izbranim nizom molekularnih označevalcev in obdelave podatkov je mono učinkovito preverjati obstoječe rodovniške podatke in napovedati očetovstvo jagnjetom v tropih, kjer pripuščajo večje število ovnov. Rezultati take analize so lahko v pomoč rejcem in selekcijskim službam za izboljšanje točnosti rodovniških podatkov in učinkovitejše načrtovanje reje in selekcije pri ovcah.

Keywords

ovce;poreklo;očetovstvo;molekularna genetika;genetski označevalci;

Data

Language: Slovenian
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL BF - Biotechnical Faculty
Publisher: Biotehniška fakulteta
UDC: 636.3:575
COBISS: 2545800 Link will open in a new window
ISSN: 1581-9175
Parent publication: Acta agriculturae Slovenica
Views: 1960
Downloads: 286
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Other data

Secondary language: English
Secondary title: Molecular genetics markers used for parentage verification and paternity determination in multiple-sire sheep pedigrees
Secondary abstract: Pedigree data from recorded flocks are of importance for the proper flock management as well as for the selection programmes and accurate performance prediction. In this study we examined if the use of eight molecular markers and computer analysis package ATLAS can be applied to verify known pedigrees of Jezersko-Solčava and Improved Jezersko-Solčava sheep breed from Slovenia. 90.9 % of pedigree data were in concordance with molecular genetic tests whereas in four pedigrees discordant parentage tests were obtained. In two cases, a different father or mother was assigned, whereas in the other two pedigrees both parents were discordant with molecular test results. In the second part of this study we aimed to determine the paternity for four lambs of Istrian Pramenka breed, in which a random mating scheme with 20 rams was used and hence the lambs had no father assigned. Using the same set of eight microsatellite markers, we were able to unequivocally determine paternity for 3 out of 4 lambs. In one case the analysis was not informative enough but with inclusion of 4 more microsatellite markers its sire could be determined. With the chosen set of microsatellite markers and data analysis programme ATLAS it is therefore possible to efficiently perform pedigree data validation as well as paternity prediction for lambs from flocks, where a large number of rams are used in a random mating system. Applying such molecular tests could help sheep breeders in flock management and improve efficiency of selection programmes.
Secondary keywords: sheep;parentage;paternity;molecular genetics;genetics markers;
URN: URN:NBN:SI
Type (COBISS): Not categorized
Pages: str. 121-131
Volume: ǂLetn. ǂ94
Issue: ǂšt. ǂ2
Chronology: 2009
Keywords (UDC): applied sciences;medicine;technology;uporabne znanosti;medicina;tehnika;agriculture and related sciences and techniques;forestry;farming;wildlife exploitation;kmetijstvo ter sorodne vede in tehnologije;animal husbandry and breeding in general;livestock rearing;breeding of domestic animals;živinoreja na splošno;vzreja domačih živali;small ruminants;mali prežvekovalci;drobnica;ovce;koze;mathematics;natural sciences;naravoslovne vede;matematika;biological sciences in general;biologija;general genetics;general cytogenetics;splošna genetika;splošna citogenetika;
ID: 21448