Žiga Jelen (Author), Janez Kovač (Author), Rebeka Rudolf (Author)

Abstract

This study reports on the successful conjugation of SARS-CoV-2 S1 spike protein fragments with gold nanoparticles (AuNPs) that were synthesised with Ultrasonic Spray Pyrolysis (USP). This method enables the continuous synthesis of AuNPs with a high degree of purity, round shapes, and the formation of a surface that allows various modifications. The conjugation mechanism of USP synthesized AuNPs with SARS-CoV-2 S1 spike protein fragments was investigated. A gel electrophoresis experiment confirmed the successful conjugation of AuNPs with SARS-CoV-2 S1 fragments indirectly. X-ray Photoelectron Spectroscopy (XPS) analysis confirmed the presence of characteristic O1s and N1s peaks, which indicated that specific binding between AuNPs and SARS-CoV-2 S1 spike protein fragments takes place via a peptide bond formed with the citrate stabiliser. This bond is coordinated to the AuNP’s surface and the N-terminals of the protein, with the conjugate displaying the expected response within a prototype LFIA test. This study will help in better understanding the behaviour of AuNPs synthesised with USP and their potential use as sensors in colorimetric or electrochemical sensors and LFIA tests.

Keywords

nanodelci zlata;ultrazvočna razpršilna piroliza;konjugacija;SARS-CoV-2 S1;karakterizacija;gold nanoparticles;ultrasonic spray pyrolysis;conjugation;characterisation;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FS - Faculty of Mechanical Engineering
Publisher: MDPI
UDC: 669.2:620.3
COBISS: 159776515 Link will open in a new window
ISSN: 2079-4991
Views: 204
Downloads: 9
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: nanodelci zlata;ultrazvočna razpršilna piroliza;konjugacija;SARS-CoV-2 S1;karakterizacija;
Type (COBISS): Article
Pages: str. 1-14
Volume: ǂVol. ǂ13
Issue: ǂiss. ǂ15, [article no.] 2160
Chronology: Aug. 2023
DOI: 10.3390/nano13152160
ID: 21520813