Nada Elmerhi (Author), Khadega Al-Maqdi (Author), Khawlah Athamneh (Author), Abdul Khayum Mohammed (Author), Tina Škorjanc (Author), Felipe Gándara (Author), Jesus Raya (Author), Pascal Simon (Author), Olivier Siri (Author), Ali Trabolsi (Author)

Abstract

Efficient enzyme immobilization is crucial for the successful commercialization of large-scale enzymatic water treatment. However, issues such as lack of high enzyme loading coupled with enzyme leaching present challenges for the widespread adoption of immobilized enzyme systems. The present study describes the development and bioremediation application of an enzyme biocomposite employing a cationic macrocycle-based covalent organic framework (COF) with hierarchical porosity for the immobilization of horseradish peroxidase (HRP). The intrinsic hierarchical porous features of the azacalix[4]arene-based COF (ACA-COF) allowed for a maximum HRP loading capacity of 0.76 mg/mg COF with low enzyme leaching (<5.0%). The biocomposite, HRP@ACA-COF, exhibited exceptional thermal stability (~200% higher relative activity than the free enzyme), and maintained ~60% enzyme activity after five cycles. LCMSMS analyses confirmed that the HRP@ACA-COF system was able to achieve >99% degradation of seven diverse types of emerging pollutants (2-mercaptobenzothiazole, paracetamol, caffeic acid, methylparaben, furosemide, sulfamethoxazole, and salicylic acid)in under an hour. The described enzyme-COF system offers promise for efficient wastewater bioremediation applications.

Keywords

covalent organic frameworks;enzymes;emerging pollutants;water purification;biocomposite;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UNG - University of Nova Gorica
UDC: 54
COBISS: 161219843 Link will open in a new window
ISSN: 0304-3894
Views: 463
Downloads: 2
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Other data

Type (COBISS): Not categorized
Pages: str. 1-9
Volume: ǂVol. ǂ459
Issue: [article no.] 132261
Chronology: Oct. 2023
DOI: 10.1016/j.jhazmat.2023.132261
ID: 21843022