magistrsko delo
Katja Hrovat (Author), Urban Švajger (Reviewer), Janja Zupan (Mentor), Jelka Pohar (Co-mentor)

Abstract

Nalezljive bolezni predstavljajo enega izmed glavnih vzrokov smrti in se v današnji dobi zaradi načina življenja tudi uspešno širijo. Njihove posledice pa imajo vpliv na mnoge sisteme. Mezenhimske matične/stromalne celice postajajo zaradi svojih sposobnosti samoobnavljanja, diferenciacije, protivnetnih in imunomodulatornih lastnosti vedno bolj privlačne tudi pri zdravljenju virusnih obolenj. Lastnosti teh celic so večinoma odvisne od donorja ter tkivnega vira. V tej nalogi smo nameravali določiti lastnosti MSC iz kostnega mozga mladega zdravega donorja, natančneje njihovo sposobnost proliferacije, diferenciacije in imunomodulatorne lastnosti pri vzorcu ročno gojenih celic ali celic gojenih v Quantum bioreaktorju. Za določanje sposobnosti proliferacije smo celice gojili v kulturi in vitro do odmiranja celic, za določitev sposobnosti diferenciacije smo celice v in vitro kulturi gojili v adipogenem, osteogenem in hondrogenem mediju, za test imunomodulatornih lastnosti MSC pa smo uporabili kondicioniran medij v kulturi celic Jurkat. Opazili smo, da preiskovane celice, ne glede na način predhodnega gojenja, v in vitro kulturi začnejo odmirati v deseti pasaži. Pri testu trilinijske diferenciacije se je kot najbolj uspešna izkazala adipogena diferenciacija tako pri histološkem testu kot pri meritvah izražanja adipogenih genov. Pri imunomodulatornem testu razlik v vplivu na proliferacijo med posameznimi vzorci nismo opazili. Naši rezultati kažejo, da imajo preiskovane celice kostnega mozga zaradi svojih lastnosti, podobnih MSC, potencial pri zdravljenju virusnih obolenj. Za določitev njihovih imumoodulatornih lastnostih so potrebni še nadaljnji testi na stimuliranih celicah imunskega sistema.

Keywords

diferenciacija celic;imunomodulatorne lastnosti MSC;izražanje genov;mezenhimske matične celice kostnega mozga;proliferacija celic;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [K. Hrovat]
UDC: 606:61:602.9(043.2)
COBISS: 179048707 Link will open in a new window
Views: 29
Downloads: 6
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Other data

Secondary language: English
Secondary title: Determination of proliferation, differentiation and immunomodulatory potential of bone marrow mesenchymal stem cells
Secondary abstract: Infectious diseases are one of the main causes of death and are spreading successfully in today's age due to the way of living. Their consequences have an impact on many systems. Mesenchymal stem/stromal cells are becoming more and more attractive in the treatment of viral diseases due to their self-renewal, differentiation, anti-inflammatory and immunomodulatory properties. The properties of these cells mostly depend on the donor and tissue source. In this thesis, we aimed to determine the properties of bone marrow-derived MSCs of a young healthy donor such astheir proliferation, differentiation and immunomodulatory potential in a sample of manually handled cells or cells grown in a Quantum bioreactor. To determine the proliferation capacity, the cells were grown in vitro until the cells started to die off. To determine the differentiation capacity we performed adipogenesis, osteogenesis and chondrogenesis of MSCs in vitro. To test the immunomodulatory properties of MSC, we applied conditioned media to Jurkat cell culture. We observed that the cells, regardless of the method of previous cultivation in vitro begin to die in the tenth passage. In the trilineage differentiation test, the adipogenic differentiation proved to be the most successful as shown by both, histology and the expression of the adipogenic genes. In the immunomodulatory test, we did not observe differences in the effect on proliferation between individual samples. Our results suggest that these bone marrow cells have potential for applications in viral diseases due to their MSC-like properties. Further tests on stimulated immune system cells are needed to determine their immunomodulatory properties.
Secondary keywords: bone marrow mesenchymal stem cells;cell differentiation;cell proliferation;gene expression;immunomodulatory properties of MSCs;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (XI, 85 str., [6] str. pril.))
ID: 21950116