Microfluidic electrochemical glucose biosensor with in situ enzyme immobilization

Nina Lokar (Author), Borut Pečar (Author), Matej Možek (Author), Danilo Vrtačnik (Author)

Abstract

The development and characterization of a microfluidic electrochemical glucose biosensor are presented herein. The transducer part is based on thin-film metal electrodes on a glass substrate. The biological recognition element of the biosensor is the pyrroloquinoline quinone–glucose dehydrogenase (PQQ-GdhB) enzyme, selectively in situ immobilized via microcontact printing of a mixed self-assembling monolayer (SAM) on a gold working electrode, while the microfluidic part of the device comprises microchannel and microfluidic connections formed in a polydimethylsiloxane (PDMS) elastomer. The electrode properties throughout all steps of biosensor construction and the biosensor response to glucose concentration and analyte flow rate were characterized by cyclic voltammetry and chronoamperometry. A measurement range of up to 10 mM in glucose concentration with a linear range up to 200 µM was determined. A detection limit of 30 µM in glucose concentration was obtained. Respective biosensor sensitivities of 0.79 nA/µM/mm$^2$ and 0.61 nA/µM/mm$^2$ were estimated with and without a flow at 20 µL/min. The developed approach of in situ enzyme immobilization can find a wide number of applications in the development of microfluidic biosensors, offering a path towards continuous and time-independent detection.

Keywords

mikrofluidni biosenzor glukoze;elektrokemični biosenzor;tankoplastna elektroda;imobilizacija encima in situ;kronoamperometrija;ciklična voltamerija;microfluidic glucose biosensor;electrochemical biosensor;thin film electrode;enzyme in situ immobilization;chronoamperometry;cyclic voltammetry;

Data

Language:	English
Year of publishing:	2023
Typology:	1.01 - Original Scientific Article
Organization:	UL FE - Faculty of Electrical Engineering
UDC:	602.1:53.082.9
COBISS:	145284355
ISSN:	2079-6374
Views:	37
Downloads:	7
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	mikrofluidni biosenzor glukoze;elektrokemični biosenzor;tankoplastna elektroda;imobilizacija encima in situ;kronoamperometrija;ciklična voltametrija;
Type (COBISS):	Article
Pages:	str. 1-16
Volume:	ǂVol. ǂ13
Issue:	ǂiss. ǂ3, [article no.] 364
Chronology:	Mar. 2023
DOI:	10.3390/bios13030364
ID:	22583478