Cysteine cathepsins, stefins and extracellular matrix degradation during invasion of transformed human breast cell lines

Irena Zajc (Author), Aleš Bervar (Author), Tamara Lah Turnšek (Author)

Abstract

Background. Human breast cellular model, comprising four cell lines originating from spontaneously immortalized human breast epithelial MCF10A cell line, its c-Ha-ras transfectant, MCF10AT, and two tumourigenic derivatives, cultured from two sequential mouse xenographs, MCF10AT-Ca1a and MCF10AT-Ca1d, were used to compare the relative protein concentration of cathepsins and stefins in single cells. Methods. The relative protein concentration of cathepsins and stefins in single cells was analysed by confocal microscopy, and compared to their protein expression in cell homogenates. Results. The most invasive, MCF10AT cell line contained several fold higher protein concentration of cathepsin B and increased levels of stefins, but similar levels of cathepsin L, compared with the parental MCF10A cells. This was associated with five fold higher endocytosis of Matrigel-DQ-collagen IV (DQC) and a simultaneous increase in signal overlap between DQC and cathepsin L as well as DQC and stefin B, but a decrease in that of DQC and cathepsin B overlap in the MCF10AT cells. Simultaneously, increased signal overlaps between both cathepsins and between cathepsins-stefins pairs, were observed in this cell line. Conclusions. These results suggest that the increased collagen endocytosis and degradation in theinvasive phenotype significantly affect also the subcellular localization of cysteine cathepsins and stefins. Based on these and the reports of other authors, we hypothesize that the intracellular degradation may also be assoeiated with cathepsin L, whereas cathepsin B in the ras transformed breastcells is involved in both, the intracellular and pericellular degradation of extracellular matrix during cell migration and invasion.

Keywords

breast neoplasms;tumor cells cultured;neoplasms invasiveness;cathepsins;extracellular matrix;

Data

Language:	English
Year of publishing:	2006
Typology:	1.01 - Original Scientific Article
Organization:	NIB - National Institute of Biology
Publisher:	Association of Radiology and Oncology
UDC:	577.2
COBISS:	1664335
ISSN:	1318-2099
Parent publication:	Radiology and oncology
Views:	37
Downloads:	6
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary title:	[Cisteinski katepsini, stefini in razgradnja izvenceličnega matriksa med invazijo človeških transformiranih celičnih linij raka dojke]
URN:	URN:NBN:SI
Type (COBISS):	Not categorized
Source comment:	42 ref.;
Pages:	str. 259-71
Volume:	ǂVol. ǂ40
Issue:	ǂno. ǂ4
Chronology:	2006
ID:	22918835