magistrsko delo
Teja Cesar (Author), Maša Knez Hrnčič (Mentor), Katja Andrina Kravanja (Co-mentor), Amra Perva-Uzunalić (Co-mentor)

Abstract

Namen magistrske naloge je bil optimizirati formulacijo polimernih nosilcev kot sistemov za dostavo farmacevtskih učinkovin. Cilj je bil izboljšati topnost izbranih farmacevtskih učinkovin ter doseči podaljšano sproščanje v simuliranih telesnih tekočinah. Polimerni nosilci so obetavni na področju razvoja sistemov s formuliranimi zdravili, saj lahko z ustrezno prilagoditvijo njihovih fizikalno-kemijskih lastnosti dosežemo izboljšano formuliranje in sproščanje farmacevtskih učinkovin. V sklopu laboratorijskega dela smo sintetizirali plastovite polimerne nosilce, v katere smo uspešno formulirali dve različni farmacevtski učinkovini z različno polarnostjo. Izbrana polimera sta bila hitozan in alginat. S procesom superkritičnega penjenja smo izdelali tudi porozne polimerne nosilce, kjer smo kot začetni polimer uporabili polikaprolakton. Kot farmacevtski učinkovini smo izbrali ketoprofen in klindamicin hidroklorid. Izbrali smo ju glede na njuno topnost v telesnih tekočinah. Ketoprofen se uporablja za zdravljenje akutne bolečine in je slabo topen, medtem ko je klindamicin hidroklorid bolj topen v telesnih tekočinah in se uporablja kot antibiotik. Karakterizacijo formuliranih polimernih nosilcev z vsebnostjo farmacevtskih učinkovin smo izvedli z vrstično elektronsko mikroskopijo in tehniko oslabljenega totalnega odboja infrardeče spektroskopije s Fourierjevo transformacijo. Z in vitro testiranjem sproščanja smo prikazali spreminjanje vsebnosti formuliranih farmacevtskih učinkovin v polimernih nosilcih glede na čas sproščanja, pridobljene vzorce smo analizirali s tekočinsko kromatografijo visoke ločljivosti in z UV-Vis spektrofotometrijo. Rezultati so nedvoumno pokazali, da je formulacija farmacevtskih učinkovin v polimerne nosilce omogočila podaljšano sproščanje v telesnih tekočinah. Ugotovitve kažejo, da so porozni polimerni nosilci bolj primerni za sisteme dostave zdravil s podaljšanim sproščanjem v primerjavi s plastovitimi polimernimi nosilci.

Keywords

farmacevtske učinkovine;polimerni nosilci;plastoviti polimeri;superkritično penjenje;in vitro sproščanje;magistrske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [T. Cesar]
UDC: 615.015.1(043.2)
COBISS: 195532291 Link will open in a new window
Views: 96
Downloads: 16
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Other data

Secondary language: English
Secondary title: Optimisation of formulation processes for active pharmaceutical ingredients in porous polymeric carriers
Secondary abstract: The aim of the master's thesis was to prepare the optimized polymer-based formulation for drug delivery. The objective was to improve the solubility of selected drugs and to achieve their sustained release in simulated body fluids. Polymer-based carriers are promising for the development of drug delivery systems, as improved drug release can be achieved by appropriate tailoring of their physiochemical properties. The experimental work involved the synthesis of layered polymeric carriers in which two drugs with different polarities were successfully formulated. The selected polymers for the preparation of layered carriers were chitosan and alginate. Porous polymeric carriers were also prepared by supercritical CO2-assisted foaming, using polycaprolactone as the starting polymer. Ketoprofen and clindamycin hydrochloride were selected based on their polarity and solubility in body fluids. Ketoprofen is anti-inflammatory drug used to treat acute pain and is poorly soluble in water-based media, while clindamycin hydrochloride is used as an antibiotic and is freely soluble in body fluids. The characterization of the formulated drug-loaded polymeric carriers was carried out using Scanning Electron Microscopy and Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy. In vitro drug release tests were used to evaluate the percentage of the drugs released from polymer carriers as a function of time. The concentrations of released drugs were determined using High-Performance Liquid Chromatography and UV-Vis spectrophotometry. The results showed that the formulation of drug-loaded polymeric carriers enabled prolonged drug release into simulated body fluids. In addition, it was shown that the porous polymeric carriers are better suited for sustained-release drug delivery systems compared to layered polymer carriers.
Secondary keywords: drugs;polymer carriers;layered polymers;supercritical foaming;in vitro drug release;
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: 1 spletni vir (1 datoteka PDF (X, 50 f.))
ID: 23108212