ǂa ǂsingle institution experience
Ira Koković (Author), Barbara Jezeršek Novaković (Author), Petra Cerkovnik (Author), Srdjan Novaković (Author)

Abstract

Background. Clonality determination in patients with lymphoproliferative disorders can improve the final diagnosis.The aim of our study was to evaluate the applicative value of standardized BIOMED-2 gene clonality assay protocolsfor the analysis of clonality of lymphocytes in a group of different lymphoid proliferations.Materials and methods. With this purpose, 121 specimens from 91 patients with suspected lymphoproliferationssubmitted for routine diagnostics from January to December 2011 were retrospectively analyzed. According to thefinal diagnosis, our series comprised 32 cases of B-cell lymphomas, 38 cases of non-Hodgkins T-cell lymphomas and51 cases of reactive lymphoid proliferations. Clonality testing was performed using the BIOMED-2 clonality assays.Results. The determined sensitivity of the TCR assay was 91.9%, while the sensitivity of the IGH assay was 74.2%. Thedetermined specificity of the IGH assay was 73.3% in the group of lymphomas and 87.2% in the group of reactivelesions. The determined specificity of the TCR assay was 62.5% in the group of lymphomas and 54.3% in the group ofreactive lesions.Conclusions. In the present study, we confirmed the utility of standardized BIOMED-2 clonality assays for the detectionof clonality in a routine diagnostical setting of non-Hodgkins lymphomas. Reactions for the detection of thecomplete IGH rearrangements and reactions for the detection of the TCR rearrangements are a good choice forclonality testing of a wide range of lymphoid proliferations and specimen types while the reactions for the detectionof incomplete IGH rearrangements have not shown any additional diagnostic value.

Keywords

Biomed-2;clonality analysis;lymphomas;IGH rearrangement;TCR rearrangement;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: OI - Institute of Oncology
Publisher: Association of Radiology and Oncology
UDC: 602.6/.7:616.1
COBISS: 1715835 Link will open in a new window
ISSN: 1318-2099
Views: 16
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Other data

Secondary language: Slovenian
Secondary keywords: klonalna anliza;limfomi;standardizirane metode;IGH preureditev;TCR preureditev;
Pages: str. 155-162
Volume: ǂVol. ǂ48
Issue: ǂno. ǂ2
Chronology: Jun. 2014
DOI: 10.2478/raon-2013-0072
ID: 23403411
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