Effects of phantom microstructure on their optical properties

Jošt Stergar (Author), Rok Hren (Author), Matija Milanič (Author)

Abstract

Significance Developing stable, robust, and affordable tissue-mimicking phantoms is a prerequisite for any new clinical application within biomedical optics. To this end, a thorough understanding of the phantom structure and optical properties is paramount. Aim We characterized the structural and optical properties of PlatSil SiliGlass phantoms using experimental and numerical approaches to examine the effects of phantom microstructure on their overall optical properties. Approach We employed scanning electron microscope (SEM), hyperspectral imaging (HSI), and spectroscopy in combination with Mie theory modeling and inverse Monte Carlo to investigate the relationship between phantom constituent and overall phantom optical properties. Results SEM revealed that microspheres had a broad range of sizes with average (13.47±5.98)μm and were also aggregated, which may affect overall optical properties and warrants careful preparation to minimize these effects. Spectroscopy was used to measure pigment and SiliGlass absorption coefficient in the VIS-NIR range. Size distribution was used to calculate scattering coefficients and observe the impact of phantom microstructure on scattering properties. The results were surmised in an inverse problem solution that enabled absolute determination of component volume fractions that agree with values obtained during preparation and explained experimentally observed spectral features. HSI microscopy revealed pronounced single-scattering effects that agree with single-scattering events. Conclusions We show that knowledge of phantom microstructure enables absolute measurements of phantom constitution without prior calibration. Further, we show a connection across different length scales where knowledge of precise phantom component constitution can help understand macroscopically observable optical properties.

Keywords

biomedicinska optika;hiperspektralno slikanje;spektroskopija;mikroskopija;tkivni fantomi;biomedical optics;hyperspectral imaging;spectroscopy;microscopy;tissue phantoms;

Data

Language:	English
Year of publishing:	2024
Typology:	1.01 - Original Scientific Article
Organization:	UL FMF - Faculty of Mathematics and Physics
UDC:	535
COBISS:	194902787
ISSN:	1083-3668
Views:	25
Downloads:	4
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	biomedicinska optika;hiperspektralno slikanje;spektroskopija;mikroskopija;tkivni fantomi;
Type (COBISS):	Article
Pages:	str. 093502-1-093502-16
Volume:	ǂVol. ǂ29
Issue:	ǂiss. ǂ9
Chronology:	2024
DOI:	10.1117/1.JBO.29.9.093502
ID:	23643057