Maša Jablonska (Author), Leonardo Cerasino (Author), Adriano Boscaini (Author), Camilla Capelli (Author), Claudia Greco (Author), Aleksandra Krivograd-Klemenčič (Author), Ute Mischke (Author), Nico Salmaso (Author), Rainer Kurmayer (Author)

Abstract

The increasing frequency of cyanobacteria blooms in waterbodies caused by ecosystem eutrophication could endanger human health. This risk can be mitigated by effective monitoring incorporating molecular methods. To date, most molecular studies on toxigenic cyanobacteria have been limited to microcystins (MCs), disregarding other cyanotoxins, to freshwater planktic habitats while ignoring benthic habitats, and to limited geographic areas (usually one or a few specific waterbodies). In this study, we used PCR-based methods including PCR product sequencing and chemical-analytical methods (LC-MS/MS) to screen many plankton (n = 123) and biofilm samples (n = 113) originating from 29 Alpine lakes and 18 rivers for their cyanotoxin production potential. Both mcyE (indicating MC synthesis) and anaC (indicating anatoxin (ATX) synthesis) gene fragments were able to qualitatively predict MC or ATX occurrence. The abundance of mcyE gene fragments was significantly related to MC concentrations in plankton samples (R2 = 0.61). mcyE gene fragments indicative of MC synthesis were most abundant in planktic samples (65 %) and were assigned to the genera Planktothrix and Microcystis. However, mcyE rarely occurred in biofilms of lakes and rivers, i.e., 4 % and 5 %, respectively, and were assigned to Microcystis, Planktothrix, and Nostoc. In contrast, anaC gene fragments occurred frequently in planktic samples (14 % assigned to Tychonema, Phormidium (Microcoleus), and Oscillatoria), but also in biofilms of lakes (49 %) and rivers (18 %) and were assigned to the genera Phormidium, Oscillatoria, and Nostocales. The cyrJ gene fragment indicating cylindrospermopsin synthesis occurred only once in plankton (assigned to Dolichospermum), while saxitoxin synthesis potential was not detected. For plankton samples, monomictic and less eutrophic conditions were positively related to mcyE/MC occurrence frequency, while oligomictic conditions were related to anaC/ATX frequency. The anaC/ATX frequency in biofilm was related to the lake habitats generally showing higher biodiversity as revealed from metabarcoding in a parallel study.

Keywords

cianotoksini;planktonski in bentoški habitati;monitoring kakovosti voda;zgodnje zaznavanje;toksigene cianobakterije;Alpe;cyanotoxins;planktic and benthic habitats;water quality monitoring;early warning;toxigenic cyanobacteria;European Alps;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 579
COBISS: 197565187 Link will open in a new window
ISSN: 0043-1354
Views: 39
Downloads: 14
Average score: 0 (0 votes)
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Other data

Secondary language: Slovenian
Secondary keywords: cianotoksini;planktonski in bentoški habitati;monitoring kakovosti voda;zgodnje zaznavanje;toksigene cianobakterije;Alpe;
Source comment: Soavtorji: Leonardo Cerasino, Adriano Boscaini, Camilla Capelli, Claudia Greco, Aleksandra Krivograd Klemenčič, Ute Mischke, Nico Salmaso, Rainer Kurmayer;
Pages: str. [1]-11
Volume: ǂVol. ǂ258
Issue: ǂ[art. no.] ǂ121783
Chronology: Jul. 2024
DOI: 10.1016/j.watres.2024.121783
ID: 23984513
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