Megan H. Cleveland (Author), Hua-Jun He (Author), Mojca Milavec (Author), Young-Kyung Bae (Author), Peter M. Vallone (Author), Jim F. Huggett (Author)

Abstract

Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials.

Keywords

digitalna PCR;digitalna verižna reakcija s polimerazo;referenčni materiali;UV-spektroskopija;digital PCR;dPCR;reference materials;UV-spectroscopy;

Data

Language: English
Year of publishing:
Typology: 1.02 - Review Article
Organization: NIB - National Institute of Biology
UDC: 577.2
COBISS: 186484995 Link will open in a new window
ISSN: 1872-9452
Views: 30
Downloads: 19
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: digitalna PCR;digitalna verižna reakcija s polimerazo;referenčni materiali;UV-spektroskopija;
Embargo end date (OpenAIRE): 2025-02-14
Source comment: Nasl. z nasl. zaslona; Opis vira z dne 22. 2. 2024;
Pages: str. 1-8
Volume: ǂVol. ǂ96
Issue: [article no.] 101256
Chronology: 2024
DOI: 10.1016/j.mam.2024.101256
ID: 23984520