Borut Šketa (Author), James L. Galman (Author), Nicholas J. Turner (Author), Polona Žnidaršič Plazl (Author)

Abstract

Enzyme immobilization is one of the essential tools that enables bioprocess intensification as it facilitates reuse of enzyme. Nanomaterials can be used as supports for enzyme immobilization because of their high surface to volume ratio. In his work a designed biocompatible non-woven nanofiber was used as a support for hexahistidine tagged amine transaminase immobilization in a microreactor. Four different nanofibers were tested and the one with surface immobilized Cu$^{2+}$ ions was proven to be the best. Additionally, direct immobilization of overexpressed enzymes in E. coli cell lysate was performed and yielded loads up to 1088 U mL$^{-1}$. The highest turnover number reached within the indicated time was 7.23 10$^6$.

Keywords

imobilizacija encimov;amin transaminaze;mikroreaktor;membrana;enzyme immobilization;amine transaminase;microreactor;membrane;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
UDC: 66.098
COBISS: 200710147 Link will open in a new window
ISSN: 1871-6784
Views: 53
Downloads: 16
Average score: 0 (0 votes)
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Other data

Secondary language: Slovenian
Secondary keywords: imobilizacija encimov;amin transaminaze;mikroreaktor;membrana;
Type (COBISS): Article
Pages: str. 46-55
Issue: ǂVol. ǂ83
Chronology: 25 Nov. 2024
DOI: 10.1016/j.nbt.2024.06.005
ID: 24524837