6N, and NMRI mice: a comparison of activation, activity, and deactivation properties in tissue slices

Viljem Pohorec (Author), Lidija Križančić Bombek (Author), Maša Skelin (Author), Jurij Dolenšek (Author), Andraž Stožer (Author)

Abstract

Although mice are a very instrumental model in islet beta cell research, possible phenotypic differences between strains and substrains are largely neglected in the scientific community. In this study, we show important phenotypic differences in beta cell responses to glucose between C57BL/6J, C57BL/6N, and NMRI mice, i.e., the three most commonly used strains. High-resolution multicellular confocal imaging of beta cells in acute pancreas tissue slices was used to measure and quantitatively compare the calcium dynamics in response to a wide range of glucose concentrations. Strain- and substrain-specific features were found in all three phases of beta cell responses to glucose: a shift in the dose-response curve characterizing the delay to activation and deactivation in response to stimulus onset and termination, respectively, and distinct concentration-encoding principles during the plateau phase in terms of frequency, duration, and active time changes with increasing glucose concentrations. Our results underline the significance of carefully choosing and reporting the strain to enable comparison and increase reproducibility, emphasize the importance of analyzing a number of different beta cell physiological parameters characterizing the response to glucose, and provide a valuable standard for future studies on beta cell calcium dynamics in health and disease in tissue slices.

Keywords

beta cell;mouse models;calcium imaging;glucose-dependence;tissue slice;

Data

Language:	English
Year of publishing:	2022
Typology:	1.01 - Original Scientific Article
Organization:	UM MF - Faculty of Medicine
Publisher:	Frontiers Research Foundation
UDC:	612.349.7
COBISS:	103005187
ISSN:	1664-2392
Views:	148
Downloads:	13
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	beta celice;mišji modeli;kalcijevo slikanje;glukozna odvisnost;tkivne rezine;
Type (COBISS):	Scientific work
Pages:	str. 1-16
Issue:	ǂVol. ǂ13
Chronology:	March 2022
DOI:	10.3389/fendo.2022.867663
ID:	24530629