Katja Kološa (Author), Aleš Leskovšek (Author), Teja Rajar (Author), Tamara Lah Turnšek (Author)

Abstract

The major obstacle to the application of mesenchymal stromal cells (MSCs) in regenerative medicine is the expansion of the donor-derived cells in vitro to obtain high cell numbers in the shortest possible time. However, MSCs gradually undergo replicative senescence after a variable number of divisions that reduce their therapeutic efficacy, which needs to be determined before administration. The authors developed a fast and simple evaluation assay testing two senescence inducers, mitoxantrone (Mxt) and trichostatin A (TSA), to predict the onset of spontaneous replicative senescence of adipose-derived mesenchymal stromal cells (ASCs) and have confirmed the correlation between induced senescence and spontaneous replicative senescence in the assay using Mxt. This protocol facilitates the standardization of therapeutic ASCs and MSCs from other origins before application.

Keywords

adipose mesenchymal stromal (stem) cells (ASCs);cell longevity;induced senescence;mesenchymal stromal (stem) cells (MSCs);mitoxantrone;replicative senescence;trichostatin A;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 577.2
COBISS: 100663043 Link will open in a new window
ISSN: 0736-6205
Views: 1
Downloads: 1
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Other data

Secondary language: Slovenian
Secondary keywords: maščobne matične celice;dolgoživost celic;mitoksantron;replikativno staranje;trihostatin A;
Pages: str. 1-10
Volume: ǂVol. ǂ72
Issue: ǂno. ǂ3
Chronology: 2022
DOI: 10.2144/btn-2021-0087
ID: 24536889