Katarina Bačnik (Author), Denis Kutnjak (Author), Silvija Černi (Author), Ana Bielen (Author), Sandra Hudina (Author)

Abstract

Crayfish are a keystone species of freshwater ecosystems and a successful invasive species. However, their pathogens, including viruses, remain understudied. The aim of this study was to analyze the virome of the invasive signal crayfish (Pacifastacus leniusculus) and to elucidate the potential differences in viral composition and abundance along its invasion range in the Korana River, Croatia. By the high-throughput sequencing of ribosomal RNA, depleted total RNA isolated from the crayfish hepatopancreas, and subsequent sequence data analysis, we identified novel and divergent RNA viruses, including signal crayfish-associated reo-like, hepe-like, toti-like, and picorna-like viruses, phylogenetically related to viruses previously associated with crustacean hosts. The patterns of reads abundance and calculated nucleotide diversities of the detected viral sequences varied along the invasion range. This could indicate the possible influence of different factors and processes on signal crayfish virome composition: e.g., the differences in signal crayfish population density, the non-random dispersal of host individuals from the core to the invasion fronts, and the transfer of viruses from the native co-occurring and phylogenetically related crayfish species. The study reveals a high, previously undiscovered diversity of divergent RNA viruses associated with signal crayfish, and sets foundations for understanding the potential risk of virus transmissions as a result of this invader’s dispersal.

Keywords

signal crayfish virome;RNA viruses;invasive alien species;invasion range;high-throughput sequencing;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 579
COBISS: 85287171 Link will open in a new window
ISSN: 1999-4915
Views: 19
Downloads: 9
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Other data

Source comment: Soavtorji: Denis Kutnjak , Silvija Černi, Ana Bielen, Sandra Hudina; Nasl. z nasl. zaslona; Opis vira z dne 17. 11. 2021; Št. članka: 2259;
Pages: str. 1-21
Volume: ǂVol. ǂ13
Issue: ǂiss. ǂ11
Chronology: 2021
DOI: 10.3390/v13112259
ID: 24556000