Tjaša Lukan (Author), Maruša Pompe Novak (Author), Špela Baebler (Author), Magda Tušek-Žnidarič (Author), Aleš Kladnik (Author), Maja Križnik (Author), Andrej Blejec (Author), Maja Zagorščak (Author), Katja Stare (Author), Barbara Dušak (Author), Anna Coll Rius (Author), Stephan Pollmann (Author), Karolina Morgiewicz (Author), Jacek Hennig (Author), Kristina Gruden (Author)

Abstract

Whereas the activation of resistance (R) proteins has been intensively studied, the downstream signaling mechanisms leading to the restriction of the pathogen remain mostly unknown. We studied the immunity network response conditioned by the potato Ny-1 gene against potato virus Y. We analyzed the processes in the cell death zone and surrounding tissue on the biochemical and gene expression levels in order to reveal the spatiotemporal regulation of the immune response. We show that the transcriptional response in the cell death zone and surrounding tissue is dependent on salicylic acid (SA). For some genes the spatiotemporal regulation is completely lost in the SA-deficient line, whereas other genes show a different response, indicating multiple connections between hormonal signaling modules. The induction of NADPH oxidase RBOHD expression occurs specifically on the lesion border during the resistance response. In plants with silenced RBOHD, the functionality of the resistance response is perturbed and the spread of the virus is not arrested at the site of infection. RBOHD is required for the spatial accumulation of SA, and conversely RBOHD is under the transcriptional regulation of SA. Using spatially resolved RNA-seq, we also identified spatial regulation of an UDP-glucosyltransferase, another component in feedback activation of SA biosynthesis, thus deciphering a novel aspect of resistance signaling.

Keywords

rastline;patologija;virusi;odpornost;immune signaling network;NADPH oxidase RBOHD;reactive oxygen species;salicylic acid;Sola-num tuberosum (potato);spatiotemporal response analysis;virus resistance;Potyvirus;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 632
COBISS: 27546883 Link will open in a new window
ISSN: 0960-7412
Views: 20
Downloads: 4
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Other data

Secondary language: Slovenian
Secondary keywords: rastline;patologija;virusi;odpornost;
Pages: str. 645-661
Volume: ǂVol. ǂ104
Issue: ǂiss. ǂ3
Chronology: 2020
DOI: 10.1111/tpj.14953
ID: 24574227