Cell proliferation on polyethylene terephthalate treated in plasma created in SO[sub]2/O[sub]2 mixtures
Abstract
Samples of polymer polyethylene terephthalate were exposed to a weakly ionized gaseous plasma to modify the polymer surface properties for better cell cultivation. The gases used for treatment were sulfur dioxide and oxygen of various partial pressures. Plasma was created by an electrodeless radio frequency discharge at a total pressure of 60 Pa. X-ray photoelectron spectroscopy showed weak functionalization of the samples’ surfaces with the sulfur, with a concentration around 2.5 at %, whereas the oxygen concentration remained at the level of untreated samples, except when the gas mixture with oxygen concentration above 90% was used. Atomic force microscopy revealed highly altered morphology of plasma-treated samples; however, at high oxygen partial pressures this morphology vanished. The samples were then incubated with human umbilical vein endothelial cells. Biological tests to determine endothelialization and possible toxicity of the plasma-treated polyethylene terephthalate samples were performed. Cell metabolic activity (MTT) and in vitro toxic effects of unknown compounds (TOX) were assayed to determine the biocompatibility of the treated substrates. The biocompatibility demonstrated a well-pronounced maximum versus gas composition which correlated well with development of the surface morphology.
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Data
| Language: | English |
|---|---|
| Year of publishing: | 2017 |
| Typology: | 1.01 - Original Scientific Article |
| Organization: | NIB - National Institute of Biology |
| UDC: | 533 |
| COBISS: |
30276647
|
| ISSN: | 2073-4360 |
| Views: | 153 |
| Downloads: | 143 |
| Average score: | 0 (0 votes) |
| Metadata: |
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Other data
| Source comment: | Nasl z nasl. zasl.; Opis vira z dne 27. 2. 2017; |
|---|---|
| Pages: | str. 82-1-82-16 |
| Volume: | ǂVol. ǂ9 |
| Issue: | ǂno. ǂ3 |
| Chronology: | 2017 |
| DOI: | 10.3390/polym9030082 |
| ID: | 24592880 |
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