Extraction of DNA from different sample types - a practical approach for GMO testing

Jana Žel (Author), Tina Demšar (Author), Dejan Štebih (Author), Mojca Milavec (Author), Kristina Gruden (Author)

Abstract

Current methods based on DNA targets for the detection, identification and quantification of genetically modified organisms (GMOs) involve extraction of the DNA. Different extraction procedures have been developed for the great variety of samples from food, feed, seeds and particular plant parts. This makes the operation of routine analytical laboratories complex and workloads heavy. Here we present a decision-making system, developed over many years of GMO testing on different samples, that result in the application of only a few extraction methods for the majority of samples. Developed decision-making system enables quicker and more cost effective testing of GMOs. In addition, the performance of DNA extraction resulting from the use of the selected extraction methods is presented for use in subsequent testing of GMOs by real time PCR methods. This approach can be used as a model for similar systems based on nucleic acid analysis in food, feed, seeds and plants.

Keywords

extraction methods;genetically modified organisms;GMO;decision- making system (biology);GMO testing;cetyltrimethylammonium bromide;CTAB;

Data

Language:	English
Year of publishing:	2015
Typology:	1.01 - Original Scientific Article
Organization:	NIB - National Institute of Biology
UDC:	604.6
COBISS:	3752783
ISSN:	1408-3671
Views:	111
Downloads:	97
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary title:	Ekstrakcija DNA iz različnih vrst vzorcev - praktični pristop za določanje GSO
Secondary abstract:	Metode za določanje, identifikacijo in kvantifikacijo gensko spremenjenih organizmov (GSO) temeljijo na zaznavanju značilnih zaporedij DNA, zato je ključni del metode ekstrakcija DNA. Za ekstrakcijo DNA iz različnih vrst vzorcev, kot so živila, krma, semena in deli rastlin, so razviti različni postopki. Delo rutinskih laboratorijev je zato zelo kompleksno in obsežno. Tu predstavljamo sistem odločanja, ki smo ga razvili v mnogih letih testiranja GSO v različnih vzorcih. Z uporabo nekaj izbranih metod ekstrakcije lahko analiziramo večino vzorcev na hitrejši in finančno učinkovitejši način. Dodatno podajamo informacijo o uporabi izbranih ekstrakcijskih metod v povezavi z nadaljnjo analizo GSO s PCR v realnem času. Ta pristop se lahko uporabi kot model za podobne sisteme, ki temeljijo na analizi nukleinskih kislin v živilih, krmi, semenih in rastlinah.
Secondary keywords:	metode ekstrakcije;gensko spremenjeni organizmi;GSO;sistem odločanja (biologija);GSO testiranje;cetiltrimetilamonijev bromid;
Pages:	str. 61-75
Volume:	ǂVol. ǂ58
Issue:	ǂšt. ǂ2
Chronology:	2015
ID:	24637841