ǂThe ǂGMOseek matrix

ǂa ǂdecision support tool for optimizing the detection of genetically modified plants

Annette Block (Author), Frédéric Debode (Author), Lutz Grohmann (Author), Julie Hulin (Author), Isabel Taverniers (Author), Linda Kluga (Author), Ulrich Busch (Author), Nancy Roosens (Author), Erik Janssen (Author), Jana Žel (Author), Kristina Gruden (Author), Dany Morisset (Author), Elodie Barbau-Piednoir (Author), Sylvia Broeders (Author), Ingrid Huber (Author), Marc Van den Bulcke (Author), Petra Heinze (Author), Gilbert Berben (Author)

Abstract

Background Since their first commercialization, the diversity of taxa and the genetic composition of transgene sequences in genetically modified plants (GMOs) are constantly increasing. To date, the detection of GMOs and derived products is commonly performed by PCR-based methods targeting specific DNA sequences introduced into the host genome. Information available regarding the GMOs’ molecular characterization is dispersed and not appropriately organized. For this reason, GMO testing is very challenging and requires more complex screening strategies and decision making schemes, demanding in return the use of efficient bioinformatics tools relying on reliable information. Description The GMOseek matrix was built as a comprehensive, online open-access tabulated database which provides a reliable, comprehensive and user-friendly overview of 328 GMO events and 247 different genetic elements (status: 18/07/2013). The GMOseek matrix is aiming to facilitate GMO detection from plant origin at different phases of the analysis. It assists in selecting the targets for a screening analysis, interpreting the screening results, checking the occurrence of a screening element in a group of selected GMOs, identifying gaps in the available pool of GMO detection methods, and designing a decision tree. The GMOseek matrix is an independent database with effective functionalities in a format facilitating transferability to other platforms. Data were collected from all available sources and experimentally tested where detection methods and certified reference materials (CRMs) were available. Conclusions The GMOseek matrix is currently a unique and very valuable tool with reliable information on GMOs from plant origin and their present genetic elements that enables further development of appropriate strategies for GMO detection. It is flexible enough to be further updated with new information and integrated in different applications and platforms.

Keywords

genetically modified organism;GMO;GMO screening;matrix approach;genetically modified plant;

Data

Language:	English
Year of publishing:	2013
Typology:	1.01 - Original Scientific Article
Organization:	NIB - National Institute of Biology
UDC:	581.1
COBISS:	2916431
ISSN:	1471-2105
Views:	30
Downloads:	26
Average score:	0 (0 votes)
Metadata:

Other data

Source comment:	Opis vira z dne 2. 10. 2013;
Pages:	str. [1-14], 256
Issue:	ǂVol. ǂ14
Chronology:	2013
DOI:	10.1186/1471-2105-14-256
ID:	24644255