Špela Alič (Author), Katarina Bačnik (Author), Tanja Dreo (Author)

Abstract

Dickeya fangzhongdai, an aggressive plant pathogen, causes symptoms on a variety of crops and ornamental plants including bleeding canker of Asian pear trees. Historical findings stress the need for a specific detection tool for D. fangzhongdai to prevent overlooking the pathogen or assigning it to general Dickeya spp. Therefore, a qualitative real-time PCR for specific detection of D. fangzhongdai has been developed and validated. The developed assay shows selectivity of 100%, diagnostic sensitivity of 76% and limit of detection with 95% confidence interval in plant matrices ranging from 311 to 2,275 cells/mL of plant extracts. The assay was successfully used in a retrospective survey of selected host plants of relevance to Europe and environmental niches relevant to D. fangzhongdai. Samples of potato tubers and plants, plants from the Malinae subtribe (apple, pear, quince, and Asian pear tree) and fresh surface water from Slovenia were analyzed. D. fangzhongdai was not detected in any plant samples, however, 12% of surface water samples were found to be positive.

Keywords

molekularno testiranje;diagnostika;rastlinski patogen;PCR v realnem času;raziskava;voda;molecular testing;diagnostics;plant pathogen;real-time PCR;Dickeya;survey;water;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 577
COBISS: 190578179 Link will open in a new window
ISSN: 1664-302X
Views: 49
Downloads: 59
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: molekularno testiranje;diagnostika;rastlinski patogen;PCR v realnem času;raziskava;voda;
Source comment: Nasl. z nasl. zaslona; Opis vira z dne 27. 3. 2024;
Pages: str. 1-15
Volume: ǂVol. ǂ14
Issue: ǂ[art. no.] ǂ1249955
Chronology: Feb. 2024
DOI: 10.3389/fmicb.2023.1249955
ID: 24676376