diplomsko delo
Brina Lucija Štruc (Author), Nataša Štajner (Mentor), Taja Jeseničnik (Co-mentor)

Abstract

Izkoriščanje naravnega mehanizma interference RNA rastlin in njihovih škodljivcev se je uveljavilo v obliki različnih strategij, kot sta HIGS in SIGS, za namene nadzora bolezni kmetijskih rastlin. Medtem ko metoda HIGS temelji na genetskem spreminjanju rastlin, je za uspešnost metode SIGS potreben le površinski nanos molekul RNA v obliki biopesticidov, s čimer se je mogoče izogniti regulatornim oviram na področju GSO. Metodi vključujeta prenos RNA molekul med rastlinami in škodljivci, kot so virusi, glive, žuželke, oomicete in nematode, in sta bili uspešno uporabljeni v številnih primerih. Kljub nedavnim napredkom na področju preučevanja prenosa sRNA med organizmi iz različnih kraljestev pa nekateri aspekti mehanizma ostajajo nerazumljeni, predvsem način prenosa malih RNA in njihovo delovanje v tarčnih organizmih. Odkritje in aplikacija interference RNA ter prenosa malih RNA med organizmi predstavljata pomemben korak k trajnostnemu in selektivnemu nadzoru rastlinskih škodljivcev, s tem pa potencialno omejitev uporabe kemičnih fitofarmacevtskih sredstev ter reševanje globalnega problema prehranske varnosti.

Keywords

interferenca RNA;male RNA;prenos sRNA;HIGS;SIGS;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [B. L. Štruc]
UDC: 601.4:577.21:606:632(043.2)
COBISS: 205287171 Link will open in a new window
Views: 128
Downloads: 19
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Other data

Secondary language: English
Secondary title: RNA interference and small RNA transfer between kingdoms as an innovative mechanism for disease control in agricultural plants
Secondary abstract: Exploitation of the natural RNA interference mechanism of plants and their pathogens has become established in the form of various strategies such as HIGS and SIGS, for the purpose of controlling diseases in agricultural crops. While HIGS is based on genetic modification of plants, the success of SIGS requires only a topical application of RNA molecules in the form of a biopesticide, thus making it possible to avoid regulatory hurdles in the field of GMO. Both methods involve the transfer of RNA molecules between plants and pathogens such as viruses, fungi, insects, oomycetes and nematodes, and have been successfully applied in many cases. However, despite recent advances in the study of cross-kingdom sRNA transfer, many aspects of the mechanism remain unclear, which makes further research a crucial part in achieving a stable and efficient sRNA transfer and function in target organisms. The discovery and application of RNA interference and the transfer of sRNA between organisms represents an important step towards sustainable and selective control of plant pathogens, potentially limiting the use of chemical pesticides and solving the global food security problem.
Secondary keywords: RNA interference;small RNA;sRNA transfer;HIGS;SIGS;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VI, 21 str.))
ID: 24810903