Influence of archaeal lipids isolated from Aeropyrum pernix K1 on physicochemical properties of sphingomyelin-cholesterol liposomes

Jan Kejžar (Author), Polona Mrak (Author), Ilja Gasan Osojnik Črnivec (Author), Nataša Poklar Ulrih (Author)

Abstract

We investigated the influence of archaeal lipids (C25,25) isolated from thermophilic archaeon Aeropyrum pernix K1 on physicochemical properties of liposomes comprised of egg sphingomyelin (SM) and cholesterol (CH) using fluorescence emission anisotropy, calcein release studies, dynamic light scattering, transmission electron microscopy and phase analysis light scattering. The 2 mol% addition of archaeal lipids enabled formation of small unilamellar vesicles by sonication while also having significant effect on reducing mean size, polydispersity index and zeta potential of C25,25/SM/CH vesicles. Increasing the ratio of C25,25 lipids in mixture of C25,25/SM/CH decreased lipid ordering parameter in dose dependent manner at different temperatures. We also demonstrated that adding 15 mol% C25,25 to SM/CH mixture will cause it to notably interact with fetal bovine serum which could make them a viable alternative adjuvant to synthetic ether-linked lipids in development of advanced liposomal vaccine delivery systems. The prospect of combining the proven strengths of SM/CH mixtures with the unique properties of C25,25 opens exciting possibilities for advancing drug delivery technologies, promising to yield formulations that are both highly effective and adaptable to a range of therapeutic applications.

Keywords

liposomi;lipidi arhej;sfingomielin;holesterol;fizikalnokemijske lastnosti;dostavni sistemi;liposomes;archaeal lipids;sphingomyelin;cholesterol;membrane fluidity;drug delivery;

Data

Language:	English
Year of publishing:	2024
Typology:	1.01 - Original Scientific Article
Organization:	UL BF - Biotechnical Faculty
UDC:	577.35:582.233:615.014.6
COBISS:	205727235
ISSN:	0005-2736
Views:	25
Downloads:	4
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	Slovenian
Secondary keywords:	liposomi;lipidi arhej;sfingomielin;holesterol;fizikalnokemijske lastnosti;dostavni sistemi;
Type (COBISS):	Article
Pages:	str. 1-9
Volume:	ǂVol. ǂ1866
Issue:	ǂissue ǂ7, [article no.] 184374
Chronology:	Oct. 2024
DOI:	10.1016/j.bbamem.2024.184374
ID:	24866560