Uporaba iPSC v regenerativni medicini

diplomsko delo

Maks Koritnik (Author), Elvira Maličev (Mentor)

Abstract

Inducirane pluripotentne matične celice (iPSC) so ena izmed možnosti zdravljenja bolezni in poškodb, njihova varnost in učinkovitost pa se že testira v kliničnih raziskavah za zdravljenje očesnih bolezni, odpovedi srca, Parkinsonove bolezni, poškodb hrbtenjače in hrustanca, akutne bolezni presadka proti gostitelju in še nekaterih ostalih. Uporaba iPSC v regenerativni medicini ima številne prednosti, kot je npr. izogib etičnim zadržkom uporabe embrionalnih matičnih celic (ESC) ter možnost izvajanja avtologne terapije s čimer se izognemo imunskemu zavračanju. V zadnjih letih opazimo precej več kliničnih raziskav v katerih so uporabljene iPSC v primerjavi z raziskavami, kjer so uporabljene ESC. Rezultati nekaterih raziskav so obetavni, a se je kljub temu potrebno soočiti z neželenimi lastnostmi iPSC, kot so tumorogenost, imunogenost, heterogenost in različna sposobnost diferenciacije v različne celične tipe. Pridobivanje iPSC za celične terapije je v veliki meri odvisno od ustreznih laboratorijskih postopkov in analize biološkega stanja iPSC na vsakem koraku teh procesov. Poleg celičnih terapij imajo iPSC velik potencial na področju modeliranja bolezni in testiranja varnosti in učinkovitosti zdravil.

Keywords

iPSC;inducirane pluripotentne matične celice;regenerativna medicina;

Data

Language:	Slovenian
Year of publishing:	2024
Typology:	2.11 - Undergraduate Thesis
Organization:	UL BF - Biotechnical Faculty
Publisher:	[M. Koritnik]
UDC:	606:61:602.9(043.2)
COBISS:	206524675
Views:	204
Downloads:	27
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Induced pluripotent stem cell (iPSC)-based therapy
Secondary abstract:	Induced pluripotent stem cells (iPSC) are one of the new options for the treatment of diseases and injuries such as macular degeneration, Parkinson’s disease, heart failure, spine and cartilage injuries, acute graft versus host disease and some others. Their safety and efficacy are already being tested in clinical trials. The use of iPSC in regenerative medicine has many advantages such as avoiding the ethical dilemmas that appear with the use of embryonic stem cells (ESC) and the option of autologous therapies which avoid immune rejections. In recent years we can notice more clinical trials that utilise iPSC in comparison to ESC. The results of some of the clinical trials are encouraging, but still there are some unwanted properties of iPSC that we must avoid, such as tumorigenicity, immunogenicity, heterogeneity and the ability of different cells to differentiate to different cell types. The production of iPSC for cellular therapies is dependent on correct laboratory procedures and the analysis of the biological state of iPSC at each step. Besides cellular therapies, iPSC hold a great potential for disease modelling and testing the safety and efficacy of novel drugs.
Secondary keywords:	iPSC;induced pluripotent stem cells;regenerative medicine;
Type (COBISS):	Bachelor thesis/paper
Study programme:	0
Embargo end date (OpenAIRE):	1970-01-01
Thesis comment:	Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages:	1 spletni vir (1 datoteka PDF (VI, 21 str.))
ID:	24870420