Guidance document on multiplex real-time PCR methods

European Network of GMO Laboratories

Lutz Grohmann (Author), Alessandra Barbante (Author), Ronnie Eriksson (Author), Francesco Gatto (Author), Tzveta Georgieva (Author), Ingrid Huber (Author), Christopher Weidner (Author), Kamila Zdeňková (Author), Heather Owen (Author), Elena Perri (Author), Ingrid Scholtens (Author), Tereza Sovová (Author), Slawomir Sowa (Author), Dejan Štebih (Author), Julie Hulin (Author), René Köppel (Author), Ugo Marchesi (Author), Lucas Marmin (Author), Marco Mazzara (Author), Frank Narendja (Author)

Abstract

Polymerase Chain Reaction (PCR) is considered the gold standard technique for the detection and quantification of Genetically Modified (GM) food, feed and seeds. The combination of two or more PCR methods in a single reaction tube is referred to as multiplex PCR and is a powerful approach already adopted by many testing laboratories. Multiplex PCR can address the need for increased and more efficient testing for the presence of GMOs in food, feed or seeds and for the quantification of GM events. Although real-time PCR has been used for GMO testing for many years as well as for research, forensic or diagnostic purposes, a comprehensive guidance on the development and implementation of multiplex PCR is currently not available. This document has been developed by experts from the European Network of GMO Laboratories (ENGL). It provides background information on the technical aspects of multiplex PCR (mpPCR), including information on instruments, fluorescent dye selection and reaction controls. It gives guidance on how to implement a previously validated mpPCR method (i.e. method verification), as well as how to develop and validate a new mpPCR method. Guidance on criteria for the acceptance of methods, examples of how to assess these criteria and troubleshooting tips are given. Finally, a list of currently used mpPCR methods is included

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Data

Language:	English
Year of publishing:	2021
Typology:	2.12 - Final Research Report
Organization:	NIB - National Institute of Biology
Publisher:	Publications Office of the European Union
UDC:	577
COBISS:	94024963
ISBN:	978-92-76-37820-4
Views:	40
Downloads:	1100
Average score:	0 (0 votes)
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Other data

Secondary keywords:	research method;technical standard;nutrition;genetically modified organism;seed;research report;
Type (COBISS):	Fundamental research
Source comment:	Soavtorji: Barbante A, Eriksson R, Gatto F, Georgieva T, Huber I, Hulin J, Köppel R, Marchesi U, Marmin L, Mazzara M, Narendja F, Owen H, Perri E, Scholtens I, Sovová T, Sowa S, Stebih D, Weidner C, Zdeňková K.; Nasl. z nasl. zaslona; Opis vira z dne 19. 1. 2022;
Pages:	1 spletni vir (1 datoteka PDF ([62] str.))
DOI:	10.2760/243914
ID:	24892355