diplomsko delo
Lovro Laporšek (Author), Zlata Luthar (Mentor)

Abstract

Trajnostna kmetijska pridelava se sooča z mnogimi izzivi, med njimi tudi z napadi virusov, ki negativno vplivajo na kakovost in količino pridelka. V iskanju možnih rešitev so se pojavile tehnologije na osnovi RNA interference in CRISPR/Cas. RNA interferenca je mehanizem, ki razreže (virusne) dvoverižne RNA in tako onesposobi razmnoževanje virusa, s CRISPR/Cas pa se lahko izzove mutacije v dovzetnostnih genih gostiteljske rastline ter s tem onemogoči tarčo virusa, ali pa se povzroči prelome virusne DNA ali RNA. Najobetavnejša tehnologija je gotovo SIGS, ki deluje na osnovi RNA interference. Gre za netransgeno metodo, ki je tudi cenovno ugodna, a je potrebno optimizirati sam privzem oziroma vstop teh molekul v rastlinske celice in povečati stabilnost malih RNA po površinski aplikaciji na rastline. Pri tem so v pomoč nanodelci, ki so sposobni zaščite malih RNA, hkrati pa jim je zaradi njihove majhnosti omogočen vstop v celice. Pred komercializacijo pa bo potrebno opraviti izbor neškodljivih nanodelcev, ki ne povzročajo toksičnih učinkov na rastline in okolje. Pričakuje se, da se bo nabor malih RNA v prihodnje še povečal, trenutno mnogo obetata umetna mikro RNA in trans-aktivna siRNA.

Keywords

bioaktivne snovi;gobe;lentinan;Lentinus edodes;naravni produkti;šitake;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [L. Laporšek]
UDC: 602.3:582.28:604.4:615.277.3(043.2)
COBISS: 207399683 Link will open in a new window
Views: 76
Downloads: 15
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Other data

Secondary language: English
Secondary title: Plant virus resistance with RNA technologies
Secondary abstract: Sustainable agricultural production faces many challenges, including viral attacks that significantly affect the quality and quantity of the crop. In the search for possible solutions, technologies based on RNA interference and CRISPR/Cas have emerged. RNA interference is a mechanism that cuts (viral) double-stranded RNA and thus disables the virus's reproduction, while CRISPR/Cas can induce mutations in the susceptibility genes of the host plant, thereby eliminating the virus target, or vaccinate viral DNA or RNA. The most promising technology is undoubtedly SIGS, which operates on the basis of RNA interference. It is a non-transgenic method that is also cost-effective, but the uptake or entry of these molecules into plant cells and the stability of small RNAs after surface application to plants need to be optimized. Nanocarriers, which can protect small RNAs and allow entry into cells due to their small size, are helpful in this regard. However, the selection of harmless nanocarriers that do not cause toxic effects on plants and the environment will be necessary before commercialization. It is expected that the repertoire of small RNAs will expand in the future, with artificial microRNAs and trans-acting siRNAs showing much promise at the moment.
Secondary keywords: bioactive compounds;lentinan;Lentinus edodes;mushrooms;natural products;shiitake;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VIII, 21 str.))
ID: 24956242