diplomsko delo
Naja Cunk (Author), Jana Murovec (Mentor), Nataša Hojnik (Co-mentor)

Abstract

Hladna plazma pri atmosferskem tlaku (ang. cold atmospheric pressure plasma, CAP) je zelena tehnologija, ki predstavlja obetavno rešitev za izboljšanje lastnosti rastlin, ob vse pogostejših naravnih nesrečah in potrebah po hrani. Plazma je opredeljena kot četrto agregatno stanje in nastane, ko nevtralne molekule zraka izpostavimo močnemu električnemu polju. Pri tem pride do ionizacije, disociacije in vzbujanja plinskih molekul. Hladna plazma, ki nastane s pomočjo električne energije iz plinskih molekul zraka, ima posebne fizikalno-kemijske lastnosti, kot so prisotnost močnega električnega polja, plazemsko proizvedenih UV fotonov in zelo reaktivnih kisikovih in dušikovih vrst. Glavni namen diplomskega dela je bil ugotoviti učinke CAP na adventivno regeneracijo poganjkov afriške vijolice (Saintpaulia ionantha L.) in tobaka (Nicotiana tabacum L.) in v ta namen odkriti najbolj primerne pogoje razelektritve, ki bodo spodbujali njihovo rast in diferenciacijo. Tekom poizkusa smo pripravili listne izsčke, jih inokulirali na pripravljena gojišča in tretirali s CAP. Pri slednjem koraku smo imeli 7 časov izpostavitve (0 s, 5 s, 15 s, 30 s, 60 s, 120 s, 240 s) ter visoko in nizko razelektritveno moč plazme. Izmerili smo tudi pH gojišča ter naredili optično emisijsko spektroskopijo in Fourierjevo transformirano infrardečo spektroskopijo plazemske razelektritvene oziroma plinske faze. Po enomesečni inkubaciji smo vsak listni izseček stehtali in mu prešteli regenerirane poganjke in korenine. Rezultati so pokazali, da plazma spodbuja delitev somatskih celic lista in nastanek kalusa, vendar samo pri tobaku. Prav tako je plazma imela vpliv na pH gojišča. Pozitivni učinek plazme na organogenezo poganjkov se je pojavil samo pri enem razelektritvenem pogoju (5 s, nizka razelektritvena moč). Pri ostalih pogojih ni prišlo do statistično značilnih razlik v primerjavi s kontrolo.

Keywords

plazma;tkivne kulture;RONS;listni izsečki;adventivna regeneracija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [N. Cunk]
UDC: 602.4:58.086.83:581.143.5(043.2)
COBISS: 207146243 Link will open in a new window
Views: 40
Downloads: 12
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Other data

Secondary language: English
Secondary title: Influence of various cold atmospheric plasma discharge conditions on adventitious regeneration of plant tissue cultures
Secondary abstract: Cold atmospheric plasma is a green technology that represents a good solution for improving plant properties, in the face of increasingly frequent natural disaster and growing population. Plasma is defined as the fourth state of matter. It is created when gas is exposed to a strong electric field, which leads to ionization, dissociation and excitation of gaseous molecules. Cold atmospheric pressure plasma (CAP) generated from air possesses unique properties, such as strong electric field, plasma produced UV photons and mostly high concentrations of reactive oxygen and nitrogen species. The main purpose of the present research was to investigate the effects of CAP on the adventitious regeneration of leaf explants from african violets (Saintpaulia ionantha L.) and tobacco (Nicotiana tabacum L.) and to identify the most optimal plasma treatment parameters to enhance their growth and differentiation. The research involved the preparation of leaf explants, inoculation of explants on culture media and their exposure to air plasma effluent for different time durations (0 s, 15 s, 30 s, 60 s, 120 s and 240 s). Two different discharge regimes were used: low and high discharge power. We also measured the pH of medium and did optical emission spectroscopy and Fourier transform infrared spectroscopy of plasma discharge and gaseous phase, respectively. After one month of incubations, the weight of the cultured leaf explants was measured and the number of developed shoots and roots counted. The results showed that plasma stimulated callus formation, but only in tobacco. The data also showed that plasma has an influence on medium pH and positive effect on the organogenesis of the shoots, but only under one discharge conditions (5 s, low discharge power). In the other conditions there were no statistically significant differences compared to the control.
Secondary keywords: plasma;tissue culture;RONS;leaf explants;adventitious regeneration;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Oddelek za agronomijo
Pages: 1 spletni vir (1 datoteka PDF (VI, 20 str.)
ID: 24956248