Sodobne transportne oblike za formulacijo aktivnih substanc v kozmetični industriji

magistrsko delo

Anja Košak (Author), Mateja Primožič (Mentor), Maja Leitgeb (Co-mentor), Nika Kučuk (Co-mentor)

Abstract

Liposomi, sferični mehurčki s fosfolipidno membrano in omogočajo enkapsulacijo različnih snovi z nadzorovanim sproščanjem. Zato so obetavni za različne aplikacije, vključno s kozmetično industrijo. Polisaharidi, naravni polimeri monosaharidov, so cenjeni v kozmetiki zaradi svojih vlažilnih in teksturnih lastnosti. Polisaharidna prevleka na liposomih lahko poveča njihovo stabilnost, zagotovi ciljno dostavo in nadzorovano sproščanje enkapsuliranih učinkovin. V magistrski nalogi smo sintetizirali liposome s polisaharidnimi prevlekami in vgrajenim ekstraktom mangovih olupkov (MNG). Uporabili smo polisaharide alginat, biosaharid gumi-1 in tara gumi ter proučevali vpliv koncentracije posameznega polisaharida in čas tvorbe prevleke na karakteristike pridobljene liposomske formulacije. Določevali smo zeta potencial, velikost delcev, polidisperzni indeks (PDI), učinkovitost enkapsulacije MNG in kinetiko sproščanja MNG iz liposomov. Rezultati so pokazali, da se povprečna velikost delcev povečuje tako z naraščajočim časom tvorbe polisaharidne prevleke, kot tudi z naraščajočo koncentracijo testiranega polisaharida. Zeta potencial vseh liposomskih formulacij brez ali s polisaharidnimi prevlekami je bil izven območja –30 mV in +30 mV, kar potrjuje, da so bile vse pripravljene formulacije stabilne. Višje koncentracije polisaharidov so povzročile manjšo monodisperznost zaradi debelejše prevleke. Pri višjih koncentracijah polisaharidov so bili povprečni premeri delcev višji zaradi tvorbe debelejše prevleke, prav tako so bile vrednosti PDI višje, kar nakazuje na manj enakomerno velikostno porazdelitev. Izmed testiranih polisaharidnih prevlek za stabilizacijo liposomov smo najboljše karakteristike za potencialno uporabo v kozmetični industriji dosegli z alginatno prevleko, in sicer s koncentracijo 8 mg/mL. Pri tem smo potrdili stabilno formulacijo funkcionaliziranih liposomov z enakomerno velikostno porazdelitvijo delcev s povprečnim premerom 2483,76 nm. Učinkovitost enkapsulacije MNG v liposome prevlečene z alginatno prevleko je znašala 59,45 % Liposomi z alginatno prevleko so izkazovali najboljši profil sproščanja MNG, pri čemer se je iz njih po 24 h sprostilo 97,47 % MNG. Prav tako je losjon z liposomi z vgrajenim MNG z alginatno prevleko izkazal najboljše inhibitorne lastnosti proti testiranim bakterijam Escherichia coli in Staphylococcus aureus ter najnižje vrednosti peroksidnega števila, kar nakazuje na uspešni zaviralni učinek oksidacije maščob v pripravljenem losjonu. Tako liposomi z vgrajenim MNG in alginatno prevleko predstavljajo sodobno transportno obliko za kozmetične aplikacije.

Keywords

mangov ekstrakt;liposomi;enkapsulacija;sproščanje;inhibitorne lastnosti;magistrske naloge;

Data

Language:	Slovenian
Year of publishing:	2024
Typology:	2.09 - Master's Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[A. Košak]
UDC:	615.014.6:602.628(043.2)
COBISS:	221666307
Views:	0
Downloads:	12
Average score:	0 (0 votes)
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Other data

Secondary language:	English
Secondary title:	Modern delivery forms for the formulation of active substances in the cosmetics industry
Secondary abstract:	Liposomes are spherical vesicles with a phospholipid membrane that enable the encapsulation of various substances with controlled release. Therefore, they are promising for various applications, including the cosmetic industry. Polysaccharides, natural polymers of monosaccharides, are valued in cosmetics for their moisturizing and textural properties. A polysaccharide coating on liposomes can enhance their stability, provide targeted delivery, and enable controlled release of encapsulated active ingredients. In the master’s thesis, we synthesized liposomes with polysaccharide coatings and embedded mango peel extract (MNG). We used the polysaccharides alginate, biosaccharide gum-1, and tara gum, and studied the influence of each polysaccharide’s concentration and coating formation time on the characteristics of the obtained liposomal formulation. We determined the zeta potential, particle size, polydispersity index (PDI), encapsulation efficiency of MNG, and release kinetics of MNG from the liposomes. The results showed that the average particle size increased with both longer coating formation time and higher concentrations of the tested polysaccharides. The zeta potential of all liposomal formulations, whether with or without polysaccharide coatings, was outside the range of -30 mV to +30 mV, confirming that all prepared formulations were stable. Higher concentrations of polysaccharides led to lower monodispersity due to a thicker coating. At higher polysaccharide concentrations, the average particle diameters were larger due to the formation of a thicker coating, and PDI values were higher, indicating a less uniform size distribution. Among the tested polysaccharide coatings for stabilizing liposomes, the best characteristics for potential use in the cosmetic industry were achieved with the alginate coating, specifically at a concentration of 8 mg/mL. This formulation demonstrated stable functionalized liposomes with a uniform particle size distribution, with an average diameter of 2483.76 nm. The encapsulation efficiency of MNG in liposomes coated with alginate was 59.45%. The alginate-coated liposomes exhibited the best release profile for MNG, with 97.47% of MNG released after 24 hours. Furthermore, a lotion with MNG-loaded liposomes with an alginate coating showed the best inhibitory properties against the tested bacteria Escherichia coli and Staphylococcus aureus, as well as the lowest peroxide values, indicating a successful inhibitory effect on lipid oxidation in the prepared lotion. Therefore, MNG-loaded liposomes with an alginate coating represent a modern delivery system for cosmetic applications.
Secondary keywords:	mango extract;liposomes;encapsulation;release;inhibitory propertiesmango extract;inhibitory properties;
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	1 spletni vir (1 datoteka PDF (XIII, 50 f.))
ID:	25006075