Rok Razpotnik (Author), Robert Vidmar (Author), Marko Fonović (Author), Damjana Rozman (Author), Tadeja Režen (Author)

Abstract

Circular RNAs (circRNAs) have been shown to play an important role in the pathogenesis of hepatocellular carcinoma (HCC). By implementing available transcriptomic analyses of HCC patients, we identified an upregulated circRNA hsa_circ_0062682. Stable perturbations of hsa_circ_0062682 in Huh-7 and SNU-449 cell lines influenced colony formation, migration, cell proliferation, sorafenib sensitivity, and additionally induced morphological changes in cell lines, indicating an important role of hsa_circ_0062682 in oncogenesis. Pathway enrichment analysis and gene set enrichment analysis of the transcriptome data from hsa_circ_0062682 knockdown explained the observed phenotypes and exposed transcription factors E2F1, Sp1, HIF-1α, and NFκB1 as potential downstream targets. Biotinylated oligonucleotide pulldown combined with proteomic analyses identified protein interaction partners of which YBX1, a known oncogene, was confirmed by RNA immunoprecipitation. Furthermore, we discovered a complex cell-type-specific phenotype in response to the oncogenic potential of hsa_circ_0062682. This finding is in line with different classes of HCC tumours, and more studies are needed to shed a light on the molecular complexity of liver cancer.

Keywords

hepatocelularni karcinom;circRNAs;transkriptomske analize;hepatocellular carcinoma;transcriptomic analyses;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 616-006
COBISS: 122291459 Link will open in a new window
ISSN: 2072-6694
Views: 13
Downloads: 0
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Other data

Secondary language: Slovenian
Secondary keywords: hepatocelularni karcinom;circRNAs;transkriptomske analize;
Type (COBISS): Article
Pages: str. 1-25
Volume: ǂVol. ǂ14
Issue: ǂno. ǂ18
Chronology: Sep. 2022
DOI: 10.3390/cancers14184524
ID: 25502424