Martin Rozman (Author), Zala Štukovnik (Author), Ajda Sušnik (Author), Amirhossein Pakseresht (Author), Matej Hočevar (Author), Damjana Drobne (Author), Urban Bren (Author)

Abstract

Humans are frequently exposed to environmental hepatotoxins, which can lead to liver failure. Biosensors may be the best candidate for the detection of hepatotoxins because of their high sensitivity and specificity, convenience, time-saving, low cost, and extremely low detection limit. To investigate suitability of HepG2 cells for biosensor use, different methods of adhesion on stainless steel surfaces were investigated, with three groups of experiments performed in vitro. Cytotoxicity assays, which include the resazurin assay, the neutral red assay (NR), and the Coomassie Brilliant Blue (CBB) assay, were used to determine the viability of HepG2 cells exposed to various concentrations of aflatoxin B1 (AFB1) and isoniazid (INH) in parallel. The viability of the HepG2 cells on the stainless steel surface was quantitatively and qualitatively examined with different microscopy techniques. A simple cell-based electrochemical biosensor was developed by evaluating the viability of the HepG2 cells on the stainless steel surface when exposed to various concentrations of AFB1 and INH by using electrochemical impedance spectroscopy (EIS). The results showed that HepG2 cells can adhere to the metal surface and could be used as part of the biosensor to determine simple hepatotoxic samples.

Keywords

biosenzorji;hepatotoksini;adhezija;nerjaveče jeklo;HepG2 cell line;impedance biosensor;adhesion;hepatotoxins;stainless steel;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: MDPI AG
UDC: 543.2/.9
COBISS: 99755011 Link will open in a new window
ISSN: 2079-6374
Views: 0
Downloads: 3
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Other data

Secondary language: Slovenian
Secondary keywords: biosenzorji;hepatotoksini;adhezija;nerjaveče jeklo;
Type (COBISS): Article
Pages: 1-15
Volume: ǂVol. ǂ12
Issue: ǂiss. ǂ3
Chronology: 2022
DOI: 10.3390/bios12030160
ID: 26197407