diplomsko delo
Matic Zorc (Author), Katarina Rudolf Pilih (Mentor)

Abstract

Resveratrol, stilbenski polifenol z močnimi antioksidativnimi, protivnetnimi, kardioprotektivnimi in nevroprotektivnimi učinki, ima velik pomen v farmacevtski, kozmetični in prehranski industriji. Vinska trta (Vitis vinifera L.) je eden od njegovih najbogatejših naravnih virov, vendar so tudi pri tej vrsti koncentracije v rastlinah pogosto omejene zaradi vplivov okolja in same razvojne faze rastlin. Kot učinkovita alternativa se vse bolj uveljavljajo suspenzijske celične kulture, ki omogočajo trajnostno, nadzorovano in celoletno proizvodnjo resveratrola z visoko čistostjo in sledljivostjo. V raziskanih študijah je bil najvišji donos, in sicer 4,18 g na liter gojišča dosežen z natančno optimizacijo pogojev rasti, kot so koncentracija biomase, zračenje, svetlobni režim ter zaporedno dodajanje specifičnih elicitorjev. Poseben izziv predstavlja prenos procesa iz laboratorijskih erlenmajeric v večje bioreaktorje, kjer je potrebno uravnotežiti mešanje, zračenje in oskrbo s hranili, kar ohranja vitalnost celic in visoko produktivnost. Celične suspenzije vključujejo tudi načela zelene biotehnologije ter zmanjšujejo okoljski odtis: proizvodnja zahteva manj prostora, manj naravnih virov (npr. vode, zemlje) in je manj obremenjujoča za okolje. Nenazadnje pa ta metoda omogoča tudi uporabo orodij metabolnega inženirstva, kar odpira možnosti za nadaljnjo izboljšavo produkcije resveratrola za specifične industrijske potrebe.

Keywords

resveratrol;trta;stilbeni;polifenoli;elicitor;suspenzije;jasmonat;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [M. Zorc]
UDC: 602.4:634.8:547.56(043.2)
COBISS: 241567235 Link will open in a new window
Views: 110
Downloads: 27
Average score: 0 (0 votes)
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Other data

Secondary language: English
Secondary title: Resveratrol production in grapevine (Vitis vinifera L.) cell suspension cultures
Secondary abstract: Resveratrol, a stilbene polyphenol with potent antioxidant, anti-inflammatory, cardioprotective, and neuroprotective properties, holds significant value in the pharmaceutical, cosmetic, and food industries. Although grapevine (Vitis vinifera L.) is one of its richest natural sources, resveratrol concentrations in plants are often limited due to environmental factors and the plant’s developmental stage. As an effective alternative, plant cell suspension cultures are increasingly being recognized for their ability to enable sustainable, controlled, and year-round production of high-purity, traceable resveratrol. In the studies reviewed, the highest yield of 4.18 g per liter of culture medium was achieved through precise optimization of growth conditions, including biomass concentration, aeration, light regime, and the sequential addition of specific elicitors. A particular challenge is the scale-up of the process from laboratory flasks to larger bioreactors, where mixing, aeration, and nutrient supply must be carefully balanced to maintain cell viability and high productivity. Cell suspension cultures also align with the principles of green biotechnology, reducing environmental impact: the production requires less space, fewer natural resources (e.g., water, land), and exerts less pressure on the environment. Moreover, this method allows for the application of metabolic engineering tools, opening new possibilities for further enhancing resveratrol production tailored to specific industrial needs.
Secondary keywords: grapevine;stilbene;polyphenols;illicitor;suspensions;jasmonate;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VI, 21 str., 1 str. pril.))
ID: 26739892