Matija Ruparčič (Author), Marko Dolinar (Author)

Abstract

Type I toxin–antitoxin (TA) systems consist of a protein toxin that exerts a cytostatic or cytotoxic effect and an antisense RNA antitoxin that prevents translation of the toxin. Although well studied, type I TA systems have so far only been discovered in bacteria from the phyla Proteobacteria, Firmicutes, and Tenericutes. We hypothesized that type I systems could also be present in Cyanobacteria. Through bioinformatic analysis of the Microcystis aeruginosa PCC 7806SL genome, we discovered ten putative type I TA loci and characterized six of them experimentally. Two of the six putative type I toxins, BH695_0320 and MsoT1 (BH695_4017), were observed to negatively affect Escherichia coli cell growth, with MsoT1 exerting a phenotype similar to SrnB, a known type I toxin. We focused on the MsoT1/MsoA1 TA system and confirmed the expression of MsoT1 and MsoA1 in our assay. Additionally, we found that MsoA1 delays the toxic effects of MsoT1, indicating its role as a cognate antitoxin of MsoT1. Our results suggest that MsoT1/MsoA1 represents a novel candidate type I TA system, the first to be discovered in the Cyanobacteria phylum.

Keywords

sistemi toksin-antitoksin tipa I;cianobakterije;Microcystis aeruginosa;bakterijski toksini;novi sistemi toksin-antitoksin;type I toxin-antitoxin systems;cyanobacteria;bacterial toxins;novel toxin-antitoxin systems;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
UDC: 577:582.232
COBISS: 243643907 Link will open in a new window
ISSN: 2072-6651
Views: 75
Downloads: 42
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: sistemi toksin-antitoksin tipa I;cianobakterije;Microcystis aeruginosa;bakterijski toksini;novi sistemi toksin-antitoksin;
Type (COBISS): Article
Pages: str. 1-25
Volume: ǂVol. ǂ17
Issue: ǂiss. ǂ8, [article no.] 360
Chronology: Aug. 2025
DOI: 10.3390/toxins17080360
ID: 26955613