diplomsko delo univerzitetnega študijskega programa I. stopnje
Nik Perko (Author), Urban Bren (Mentor), Zala Štukovnik (Co-mentor)

Abstract

Za detekcijo različnih farmacevtskih učinkovin smo uporabili impedančni biosenzor, ki je temeljil na predhodno razviti in optimizirani elektrokemijski celici z biološko komponento kvasovk vrste Saccharomyces cerevisiae. Za izdelavo elektrod smo uporabili nerjavno jeklo tipa 316, iz katerega smo sestavili trielektrodni elektrokemijski sistem v RCW side-by-side konfiguraciji. Pripravili smo različno koncentrirane raztopine acetaminofena, naproksena in izoniazida ter jih tekom meritev dodajali v zaprt elektrokemijski sistem. Stabilnost elektrokemijskega sistema smo ocenili z merjenjem potenciometrije pri odprtem tokokrogu (OCP), z elektrokemijsko impedančno spektroskopijo (EIS) pa smo spremljali odziv kvasovk vrste Saccharomyces cerevisiae na delovni elektrodi ob odsotnosti (0,9 % NaCl) in prisotnosti farmacevtskih učinkovin. Impedančni odziv sistema smo predstavili z Nyquistovimi in Bodejevimi diagrami, rezultatom pa izračunali standardni odklon, natančnost in točnost. Za vsako izmed farmacevtskih učinkovin smo oblikovali kalibracijsko krivuljo z R2 vrednostjo nad 0,95 ter izračunali mejo zaznavnosti (LOD). Za preučevanje vpliva farmacevtskih učinkovin na kvasovke vrste Saccharomyces cerevisiae smo izvedli eksperiment z rastno krivuljo, pri kateri smo spremljali spreminjanje optične gostote pri 600 nm (OD600) s časom po dodatku kontrole (0,9 % NaCl) in različno koncentriranih raztopin farmacevtskih učinkovin. Ugotovili smo, da je naš biosenzor zmožen detekcije učinka različnih koncentracij acetaminofena, naproksena in izoniazida. Dokazali smo, da z naraščanjem koncentracije naštetih farmacevtskih učinkovin pada impedanca elektrokemijskega sistema. S pripravo rastnih krivulj smo dokazali, da dodatek farmacevtskih učinkovin zavira rast celic kvasovk. Razvit biosenzor predstavlja cenovno ugodno in obetavno alternativo za hitrejšo in enostavnejšo detekcijo farmacevtskih učinkovin v vzorcih odpadne vode z uporabo živih celic kot biološke prepoznavne komponente.

Keywords

impedančni biosenzor;elektrokemijska celica;kvasovke vrste Saccharomyces cerevisiae;elektrokemijska impedančna spektroskopija;EIS;farmacevtske učinkovine;diplomske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher: [N. Perko]
UDC: 544.6:57(043.2)
COBISS: 250648323 Link will open in a new window
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Downloads: 6
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Other data

Secondary language: English
Secondary title: Detection of pharmaceutically active compounds using yeast cells of the species Saccharomyces cerevisiae applied to an electrochemical cell (biosensor)
Secondary abstract: A previously developed and optimised electrochemical cell was used as an impedimetric biosensor, utilizing yeast Saccharomyces cerevisiae as a biological recognition element for the detection of different pharmaceutically active compounds. Electrodes were made from type 316 stainless steel and were implemented in a three-electrode system in the RCW side-by-side configuration. Acetaminophen, naproxen, and isoniazid solutions of varying concentrations were prepared and applied to the closed electrochemical system. The stability of the electrochemical system was evaluated by measuring the open circuit potential (OCP), and electrochemical impedance spectroscopy (EIS) was employed for tracking the response of the yeast Saccharomyces cerevisiae on the working electrode in the absence (0,9% NaCl) and in the presence of pharmaceutically active compounds. The impedimetric response of the system was presented using Nyquist and Bode diagrams, and the results' standard deviation, precision, and accuracy were calculated. Moreover, we calculated the limit of detection (LOD) and devised a calibration curve for each of the studied compounds with R2 values above 0,95. To study the effect of pharmaceutically active compounds on the yeast Saccharomyces cerevisiae, an experiment using growth curves was conducted, which monitored the changes in optical density at 600 nm (OD600) over a specific time-period after the addition of the control (0,9% NaCl) and different concentrations of pharmaceutically active compounds. We found that the studied biosensor could detect different concentrations of acetaminophen, naproxen, and isoniazid, while confirming that the impedance of the electrochemical system decreases as the concentration of the compounds increases. Using growth curves, we confirmed that pharmaceutically active compounds lead to the growth inhibition of Saccharomyces cerevisiae yeast cells. The developed biosensor presents an interesting and affordable alternative for rapid and simplified detection of pharmaceutically active compounds in wastewater using living cells as the biological recognition element.
Secondary keywords: impedimetric biosensor;electrochemical cell;yeast Saccharomyces cerevisiae;electrochemical impedance spectroscopy (EIS);pharmaceutically active compounds;
Type (COBISS): Bachelor thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages: 1 spletni vir (1 datoteka PDF (X, 46 str.))
ID: 27038307