diplomsko delo
Živa Vilfan (Author), Monika Novak (Mentor)

Abstract

Zaradi staranja prebivalstva ter večjega števila bolnikov in imunsko oslabljenih oseb, so glivične okužbe vse pogostejše. Med povzročitelji prevladujejo kvasovke iz rodu Candida, ki zaradi povečane uporabe antimikotikov razvijajo rezistenco. Ker so tudi ekstremna okolja naravni rezervoarji potencialno patogenih gliv, je bil namen diplomskega dela ugotoviti odpornost petih vrst rodu Candida, izoliranih iz ledeniške vode, slanice in urbanih območij, proti devetim protiglivnim učinkovinam. Za določanje minimalne inhibitorne koncentracije (MIC) smo uporabili komercialno ploščo Thermo Scientific z devetimi antimikotiki. Kvasovke smo nanesli v koncentraciji 1,5 × 10⁸ CFU/mL. Rast je bila ocenjena vizualno; modra barva je pomenila, da ni rasti, roza barva da je rast dobra, prva vdolbinica, drugačna od pozitivne kontrole, pa MIC. Ehinokandini, amfotericin B in flukitozin so bili učinkoviti proti večini sevov. Največjo odpornost smo zaznali pri sevih vrste C. tropicalis, kjer so vsi sevi kazali odpornost vsaj proti enemu antimikotiku. Ti sevi so bili izolirani iz urbanega okolja, kar je posebej zaskrbljujoče, saj je C. tropicalis vse pogostejši povzročitelj okužb z naraščajočo rezistenco. Pri vrsti Candida parapsilosis smo dodatno preverjali povezavo med fenotipom kolonij in morebitno odpornostjo. Izstopal je le sev iz Mrtvega morja, ki je edini oblikoval koncentričen fenotip ter kazal visoko odpornost proti flukonazolu.

Keywords

Candida;kvasovke;antimikotiki;minimalna inhibitorna koncentracija;odpornost;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [Ž. Vilfan]
UDC: 615.28.015.8(043.2)
COBISS: 246683907 Link will open in a new window
Views: 80
Downloads: 10
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Other data

Secondary language: English
Secondary title: Resistance of Candida strains against selected antifungal agents
Secondary abstract: Due to the aging population and increasing number of immunocompromised individuals, fungal infections are on the rise. Yeasts of the genus Candida are the most common causative agents, with growing resistance linked to frequent antifungal use. As extreme environments may serve as reservoirs of potentially pathogenic fungi, this thesis investigated the resistance of five Candida species, isolated from glacial water, brine, and urban areas, against nine antifungal agents. Minimum inhibitory concentration (MIC) was determined using a commercial Thermo Scientific plate. Yeasts were applied in concentration of 1.5 × 10⁸ CFU/mL. Growth was assessed visually: blue colour indicated no growth, pink indicated good growth, and the MIC was the first well with different colour than the positive control. Echinocandins, amphotericin B, and flucytosine were effective against most strains. The highest resistance was found in Candida tropicalis, with all strains resistant to at least one agent. These strains originated from urban environment, which raises concern as C. tropicalis is increasingly present in infections. For Candida parapsilosis, we additionally explored the relationship between colony phenotype and resistance. Notably, the Dead Sea strain exhibited a unique concentric phenotype and high resistance to fluconazole. Our findings highlight the potential threat posed by environmental Candida strains.
Secondary keywords: Candida;yeast;antimycotics;minimum inhibitory concentration;resistance;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VIII, 20 str.,[1] str. pril.))
ID: 27211641