Vpliv gnojenja z dušikom na citogenetski material šalotke (Allium cepa var. ascalonicum) z različnih območij

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Language:	Slovenian
Year of publishing:	2012
Source:	Ljubljana
Typology:	2.11 - Undergraduate Thesis
Organization:	UL PEF - Faculty of Education
Publisher:	[D. Rizmal]
UDC:	58(043.2)
COBISS:	9296713
Views:	1269
Downloads:	223
Average score:	0 (0 votes)
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Secondary language:	English
Secondary title:	The impact of nitrogen fertilization on the citogenetic material of shallot (Allium cepa var. ascalonicum) from different regions
Secondary abstract:	The aim of my thesis was to assess the impact of nitrogen fertilization on citogenetic material of the biondicator plant shallot (Allium cepa L. var. ascalonicum) through cytogenetic bioindication, conducted beteen 2004 and 2005 in situ. The research was carried out in two locations in Zavodnje and Velenje as well as in the laboratory. Shallot with a diploid number of chromosomes was selected as an example of a biondicator plant due to its fast growing roots, a stable number of chromosomes, genetically uniform material, a quick response to genotoxic substances, a low number of spontaneous chromosome damage and the size of its cells with large and well visible chromosomes. The results of our research were variable. The pot experiment proved no statistically characteristic differences between various levels of fertilization in any of the locations researched. Besides, no significant differences between any of the locations were noticed. The results of our research, conducted in the laboratory on bulbs from pot experiment in Velenje, demonstrated statistically significant differences in the mitotic index between the absence of fertilization and the second level of nitrogen fertilization. Among ecotoxicological results an increased number of chromosome aberrations in the laboratory experiment was noticed. According to the results, the quantity of nitrogen fertilization exerted statistically significant effects on all chromosome aberrations. The citogenetic bioindication is an effective method to detect citotoxic and genotoxic substances to which the plants are exposed. However, considering the analysed values of mitotoxic activity and the chromosome aberrations, it is impossible to reach a simple conclusion due to a high variability of the results, insufficient samples and multi stressors to which the plants are exposed in their natural environment Owing to that it is essential to verify the cause of chromosome aberrations in micro nucleus by using more samples and different stressors in the laboratories.
Secondary keywords:	botany;botanika;
File type:	application/pdf
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. Ljubljana, Pedagoška fak., Biotehniška fak., Fak. za kemijo in kemijsko tehnologijo, Naravoslovnotehniška fak., Kemija in biologija
Pages:	X, 66 f.
Type (ePrints):	thesis
Title (ePrints):	The impact of nitrogen fertilization on the citogenetic material of shallot (Allium cepa var. ascalonicum) from different regions
Keywords (ePrints):	šalotka (Allium cepa L. var. ascalonicum)
Keywords (ePrints, secondary language):	shallot (Allium cepa L. var. ascalonicum)
Abstract (ePrints):	Z uporabo rastlinske citogenetske bioindikacije smo v letih 2004 in 2005 in situ ocenjevali vpliv gnojenja z dušikom na citogenetski material bioindikatorske rastline šalotke (Allium cepa L. var. ascalonicum). Raziskave so potekale na dveh lokacijah v Zavodnjah in v Velenju. Dodatne raziskave smo opravili še v laboratoriju. Šalotka z diploidnim številom kromosomov je bila izbrana kot bioindikatorska rastlina zaradi hitre rasti korenin, stalnega števila kromosomov, gensko enovitega materiala, hitrega odziva na genotoksične snovi, majhnega števila spontanih kromosomskih poškodb in velikosti celic z velikimi in lepo vidnimi kromosomi. Rezultati raziskav so bili zelo variabilni. V lončnem poskusu nismo ugotovili statistično značilnih razlik med nivoji gnojenja na nobeni od lokacij. Prav tako nismo ugotovili razlik med lokacijama. V laboratorijskem poskusu s čebulicami iz lončnega poskusa v Velenju smo ugotovili, da se pojavljajo statistično značilne razlike v mitotskem indeksu: med obravnavanjem brez gnojenja in drugim nivojem gnojenja z dušikom. Med ekotoksikološkimi kazalniki smo ugotovili povečano število vseh kromosomskih aberacij v laboratorijskem poskusu. Rezultati so pokazali, da količina gnojenja z dušikom statistično značilno vpliva na delež vseh kromosomskih aberacij. Metoda citogenetske bioindikacije je dobra metoda za zaznavanje citotoksičnih in genotoksičnih substanc, ki so jim rastline izpostavljene, vendar glede na analizirane vrednosti za mitototsko aktivnost in delež kromosomskih aberacij ne moremo podati enostavnih zaključkov zaradi velike variabilnosti rezultatov, razmeroma majhnega vzorca ter multistresnega vpliva, kateremu so izpostavljene rastline v naravnem okolju. Vzrok za nastanek kromosomskih aberacij in mikrojeder bi bilo smiselno preveriti z večjimi vzorci in s posameznimi stresorji v laboratorijskih razmerah.
Abstract (ePrints, secondary language):	The aim of my thesis was to assess the impact of nitrogen fertilization on citogenetic material of the biondicator plant shallot (Allium cepa L. var. ascalonicum) through cytogenetic bioindication, conducted beteen 2004 and 2005 in situ. The research was carried out in two locations in Zavodnje and Velenje as well as in the laboratory. Shallot with a diploid number of chromosomes was selected as an example of a biondicator plant due to its fast growing roots, a stable number of chromosomes, genetically uniform material, a quick response to genotoxic substances, a low number of spontaneous chromosome damage and the size of its cells with large and well visible chromosomes. The results of our research were variable. The pot experiment proved no statistically characteristic differences between various levels of fertilization in any of the locations researched. Besides, no significant differences between any of the locations were noticed. The results of our research, conducted in the laboratory on bulbs from pot experiment in Velenje, demonstrated statistically significant differences in the mitotic index between the absence of fertilization and the second level of nitrogen fertilization. Among ecotoxicological results an increased number of chromosome aberrations in the laboratory experiment was noticed. According to the results, the quantity of nitrogen fertilization exerted statistically significant effects on all chromosome aberrations. The citogenetic bioindication is an effective method to detect citotoxic and genotoxic substances to which the plants are exposed. However, considering the analysed values of mitotoxic activity and the chromosome aberrations, it is impossible to reach a simple conclusion due to a high variability of the results, insufficient samples and multi stressors to which the plants are exposed in their natural environment Owing to that it is essential to verify the cause of chromosome aberrations in micro nucleus by using more samples and different stressors in the laboratories.
Keywords (ePrints, secondary language):	shallot (Allium cepa L. var. ascalonicum)
ID:	8310625