Isolation of chlorophylls from stinging nettle (Urtica dioica L.)

Maša Hojnik (Author), Mojca Škerget (Author), Željko Knez (Author)

Abstract

This study was aimed to assess the feasibility of extracting chlorophylls fromstinging nettle (Urtica dioica L.) by solvent extraction. In preliminary, the investigation concerned three parts of raw material (leaves, stalk and whole plant), different preservation techniques (blanching and drying) and different storage temperatures of preserved material ( |20 C for blanched and 4 C for dried sample). Extracts were analysed for chlorophyll A and chlorophyll B, respectively, by means of HPLC method. Afterwards, optimizationof single-step conventional extraction was carried out by examining the influence of the following process parameters on the extraction yield and isolation efficiency of chlorophylls: type of organic solvent, content of water in ethanol/water mixtures, ratio solvent/material, temperature, time of extraction and pH of the extraction mixture. Furthermore,to improve the yield and/or the selectivity of the extraction, multi-step conventional extraction experiments as well as other solid ]liquid extraction techniques (soxhlet and ultra-sound assisted) were performed and compared. c 2007 Elsevier B.V. All rights reserved.

Keywords

kemijska procesna tehnika;ekstrakcija;koprive;izolacija klorofila;vsebnost klorofila;topila;chemical processing;extraction;nettle;isolation of chlorophyll;chlorophyll content;solvent extraction;

Data

Language:	English
Year of publishing:	2007
Typology:	1.01 - Original Scientific Article
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC:	66.061
COBISS:	11289878
ISSN:	1383-5866
Views:	2165
Downloads:	99
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary keywords:	kemijska procesna tehnika;ekstrakcija;koprive;izolacija klorofila;vsebnost klorofila;topila;
URN:	URN:SI:UM:
Pages:	str. 37-46
Volume:	ǂVol. ǂ57
Issue:	ǂiss. ǂ1
Chronology:	2007
ID:	8717346