M.P. Romero (Author), L. Calvo (Author), C. Alba (Author), Maja Leitgeb (Author), Mateja Primožič (Author), Željko Knez (Author)

Abstract

Isoamyl acetate was successfully synthesized from isoamyl alcohol in supercritical carbon dioxide by enzymatic catalysis. First, the impact of the acyl donor was investigated. Among several reactants, including acetic acid and two different acetates, acetic anhydride gave best yields. Then, two different immobilized lipases (Novozym 435 from Candida antarctica and Lipozyme RM-IM from Rhizomucor miehei) as biocatalysts for the above-mentionedreaction were compared. An esterification extent of 100% was obtained in continuous operation using acetic anhydride as acyl donor and Novozym 435 as enzyme. The amount of enzyme preparation was optimised to 6.25 g/mol alcohol. The effect of substrates load in the solvent was investigated. Operating at a CO2žsubstrates molar ratio below 7.0, the conversion of alcoholdecreased, probably due to an inhibitory effect on enzyme by high concentration of acetic anydride or by produced acetic acid. Pressure in the range of 8-30 MPa showed no effect on this reaction, while an increase in temperature (over 313 K) led to lower production of isoamyl acetate. Novozym 435 was very stable not finding any loss of activity during one month of continuous operation. Finally, carbon dioxide was compared to a conventional organic solvent (n-hexane). Initial reaction rate was higher in SC-CO2, although final esterification extent was similar in both media.

Keywords

kemijska procesna tehnika;visokotlačna tehnologija;superkritični CO2;sinteze estrov;izoamil acetat;encimska kataliza;imobilizirana lipaza;proizvodnja izoamil acetata;proizvodnja naravnih arom;chemical processing;high pressure technology;superkritical CO2;ester synthesis;isoamyl acetate;enzymatic catalysis;immobilized lipase;isoamyl acetate production;natural flavors;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC: 66.091-987
COBISS: 9162774 Link will open in a new window
ISSN: 0896-8446
Views: 1724
Downloads: 175
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Other data

Secondary language: English
Secondary keywords: kemijska procesna tehnika;visokotlačna tehnologija;superkritični CO2;sinteze estrov;izoamil acetat;encimska kataliza;imobilizirana lipaza;proizvodnja izoamil acetata;proizvodnja naravnih arom;
URN: URN:SI:UM:
Pages: str. 77-84
Volume: ǂVol. ǂ33
Issue: ǂiss. ǂ1
Chronology: 2005
ID: 8718457