Zoran Novak (Author), Maja Leitgeb (Author), Vlasta Krmelj (Author), Željko Knez (Author)

Abstract

The enzymes (lipases from Candida rugosa and porcine pancreas) were immobilized on silica aerogels by sol-gel procedure followed by supercritical drying with CO2. Such immobilized enzymes were used as biocatalysts for esterification in supercritical CO2 and near critical propane at 40 °C and 100 bar. It was found out that the initial reaction rates in propane rose two to three times in comparison with the same reaction, catalyzed by free lipase. SC CO2 deactivated the non-immobilized lipase in reaction mixture while with the immobilized enzyme the conversion was 35%. The initial reaction rates in propane were 20 times higher than in water medium due to the properties of propane as a medium for esterification of fatty acids.

Keywords

kemijska procesna tehnika;biotehnologija;esterifikacija;imobilizacija encimov;superkritični CO2;lipaze;superkritično CO2 sušenje;chemical processing;biotechnology;esterification;immobilization of enzymes;supercritial CO2;propane;lipases;supercritical CO2 drying;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC: 66.095.1
COBISS: 8156950 Link will open in a new window
ISSN: 0896-8446
Views: 1416
Downloads: 22
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Other data

Secondary language: English
Secondary keywords: kemijska procesna tehnika;biotehnologija;esterifikacija;imobilizacija encimov;superkritični CO2;lipaze;superkritično CO2 sušenje;
URN: URN:SI:UM:
Pages: str. 169-178
Volume: ǂVol. ǂ27
Issue: ǂiss. ǂ2
Chronology: 2003
ID: 8718849