Enzymatic fatty ester synthesis

Simona Pečnik (Author), Željko Knez (Author)

Abstract

Opisana je sinteza maščobnega estra z imobilizirano lipazo iz Mucor Miehei, ki je 1,3 specifična. 1,2-izopropiliden glicerol smo dobili s kondenzacijo glicerola z acetonom. Z esterifikacijo 1,2-izopropiliden glicerola in oleinove kisline v prisotnosti lipaze iz Mucor Miehei smo proizvedli 1,2-izopropiliden-3-oleil glicerol. Za določitev optimalnih pogojev za proces esterifikacije smo ugotavljali vplive raznih procesnih parametrov (temperature in tlaka) in različnih razmerij encim/substrat. Najvišja presnova oleinove kisline (80%) je bila pri 55 0C in 57,057 bar. Optimalni dodatek lipaze k substratom je bil 0,096 g na gram rakcijske zmesi. Esterifikacija je reverzibilna reakcija drugega reda. Termodinamske lastnosti smo določili pri 55 oC in o,o57 bar. Energija aktiviranja je bila 20,82vkJ/mol, entropija aktivacije -0,26 kJ/(K mol) in prosta energija aktivacije je bila 103,32 kJ/mol.

Keywords

procesna tehnika;biotehnologija;esterifikacija;sinteze;estri;lipaze;encimi;Mucor miehei;kinetika reakcij;termodinamika reakcija;1,2-izopropiliden-3-oleil glicerol;chemical engineering;biotechnology;esterification;syntheses;esters;enzymes;lipase;reaction kinetics;reaction thermodynamics;1,2-isopropylidene-3-oleoyl glycerol;

Data

Language:	English
Year of publishing:	1992
Typology:	1.01 - Original Scientific Article
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
UDC:	66.095.13:577.15
COBISS:	4025092
ISSN:	0003-021X
Views:	1773
Downloads:	81
Average score:	0 (0 votes)
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Other data

Secondary language:	English
Secondary title:	Enzymatic fatty ester synthesis.
Secondary abstract:	Fatty ester synthesis with immobilized 1,3-specific lipase from Mucor Miehei is described. 1,2-Isopropylidene glycerol was peoduced by condensation of glycerol with acetone was esterified with oleic acid in the presence of a Mucor Miehei lipaze (Lipozyme TM) to obtain 1,2 isopropylidene- 3-oleoyl glycerol. The effects of various process parameters (temperature and pressure)and various ratios (enzyme/substrate) have been investigated to determine optimal conditions for the esterification process. The highest conversion of oleic acid (80% w/w) was obtained at 55 oC and 57.057 bar, while the optimal addition of lipase to substrate was determined to be 0,096 g per gram of reaction mixture. The esterification can be modelled successfully as a reverse second order reaction. Thermodynamic properties of the reaction system at 55 oC and 0.057 bar were also determined. Activation energy was 20.82 kJ/mole, entropy of activation -0,26 kJ/(Kmole) and free energy of activation was 103.32 kJ/mole.
Secondary keywords:	procesna tehnika;biotehnologija;esterifikacija;sinteze;estri;lipaze;encimi;Mucor miehei;kinetika reakcij;termodinamika reakcija;1;2-izopropiliden-3-oleil glicerol;
URN:	URN:SI:UM:
Pages:	str. 261-265
Volume:	ǂVol. ǂ69
Issue:	ǂno ǂ3
Chronology:	Mar. 1992
ID:	8723636