Separacija fenolnih spojin iz zelišč

diplomsko delo visokošolskega strokovnega študijskega programa

Suzana Petek (Author), Mojca Škerget (Mentor), Željko Knez (Co-mentor)

Abstract

Zelišča, ki uspevajo v naših krajih sem ekstrahirala ter analizirala izkoristek ekstrakcije posameznih delov rastline (steblo, listi, cvet) in vsebnost učinkovin. Analizirala sem ekstrakte klasaste mete, koroške mete, dlakave mete, ožepka, sivke in rimske kamilice. Za pridobivanje ekstraktov sem uporabila dve ekstrakcijski metodi, konvencionalno ekstrakcijo pri kateri sem kot topilo uporabila 96% etanol in sem jo izvajala pri sobni temperaturi in pri 40°C ter superkritično ekstrakcijo s CO2 pri 280 bar in 60°C. Pripravljenim raztopinam ekstraktov sem določila vsebnost skupnih fenolov, skupnih flavonoidov ter antioksidativno aktivnost. Uporabila sem spektrofotometrijo in na podlagi dobljenih absorbanc pri različnih valovnih dolžinah izračunala % antioksidativne aktivnosti in na podlagi umeritvenih krivulj določila koncentracijo skupnih fenolov in flavonoidov. Rezultate sem podala na gram ekstrakta in na gram materiala. Antioksidativno učinkovitost ekstraktov sem določila z uporabo DPPH metode. Rezultati analiz vsebnosti antioksidativnih komponent so pokazali, da je v raztopinah ekstraktov, ki sem jih pridobila s konvencionalno ekstrakcijo, višja vsebnost učinkovin kot pa v ekstraktih dobljenih s superkritično ekstrakcijo s CO2. Vsebnost komponent je odvisna tudi od dela rastline, več učinkovin je v listih in v cvetu posameznih rastlin kot pa v steblu.

Keywords

zelišča;meta;sivka;ožepek;rimska kamilica;superkritična eksatrakcija s CO2;konvencionalna ekstrakcija;antioksidativna aktivnost;skupni fenoli;skupni flavonoidi;

Data

Language:	Slovenian
Year of publishing:	2013
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[S. Petek]
UDC:	665.53:547.913(043.2)
COBISS:	17475094
Views:	2562
Downloads:	408
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	SEPARATION PHENOLIC COMPONENTS FROM HERBS
Secondary abstract:	The herbs native to aur region were extracted and the efficiency of the extraction of individual herb parts (stem, leaf, flower) and the content of active ingredients were analysed. The extracts of spike-like mint, carinthian mint, hairy mint, hyssop, lavender and roman chamomile were analysed. Two extraction methods were used; a conventional extraction with 96% ethanol at room temperature and at 40°C and the supercritical extraction with CO2, whic was performed at 280 bar and 60°C. The content of total phenolic compounds and total flavonoids in extracts and antioxidant activity of the extracts were determined by spectrophotometer. Based on the absorbance measured at different wavelengths the % of antioxidant activity of extracts and the concentration of total phenols and flavonoids were determined. The results were expressed per g of extract and per g of material. The antioxidant activity of the extracts was determined by DPPH method. The results of the analysis of antioxidant components show that the solutions of the extracts, gained with the conventional method, have a higher content of active ingredients in comparison to supercritical extraction. The results also vary depending on the part of the plant more active ingredients were extracted from the flowers than from the stems of the plants.
Secondary keywords:	herbs;mint;lavender;hyssop;roman chamomile;supercritical extraction with CO2;conventional extraction;antioxidant activity;total phenolics;total flavonoids;
URN:	URN:SI:UM:
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	XIII, 63 str.
ID:	8726164