Algoritem za detekcijo komponent kompleksov proteinov v interakciji z RNA

Abstract

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Language:	Slovenian
Year of publishing:	2014
Typology:	2.11 - Undergraduate Thesis
Organization:	UL FRI - Faculty of Computer and Information Science
Publisher:	[M. Spasić]
UDC:	004.4:577.2(043.2)
COBISS:	1536122307
Views:	97
Downloads:	10
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Secondary language:	English
Secondary title:	Algorithm for detecting components of ribonucleoprotein complexes
Secondary abstract:	Proteins play an important role in many processes in a cell. Protein-RNA interactions greatly affect the balancing of gene expressions and consequently their functions. Interaction with RNA may occur with a single protein or a protein complex. iCLIP method is able to detect the protein-RNA crosslink spots with nucleotide resolution. We developed a method that takes a set of crosslink spots that intersect with the choosen protein complex as an input. Method is searching the crosslink spots neighbourhood for the protein-RNA interaction patterns. Found patterns include parts (proteins) of the choosen complex and other proteins that are not a part of the choosen complex, but still affect the interactions of the choosen complex with the RNA. Method that we developed is based on the non-negative matrix factorization. We successfully tested the method on a SmB complex where we found a few proteins that cooporate with SmB complex.
Secondary keywords:	protein-RNA interactions;nonnegative matrix factorization;iCLIP;protein complexes;computer science;computer and information science;diploma;
File type:	application/pdf
Type (COBISS):	Bachelor thesis/paper
Study programme:	1000468
Thesis comment:	Univ. v Ljubljani, Fak. za računalništvo in informatiko
Pages:	50 str.
ID:	8739387